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Katnb1tm1a(EUCOMM)Hmgu
Targeted Allele Detail
Summary
Symbol: Katnb1tm1a(EUCOMM)Hmgu
Name: katanin p80 (WD40-containing) subunit B 1; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
MGI ID: MGI:4436305
Gene: Katnb1  Location: Chr8:95807804-95826502 bp, + strand  Genetic Position: Chr8, 47.12 cM, cytoband C5
Alliance: Katnb1tm1a(EUCOMM)Hmgu page
IMPC: Katnb1 gene page
Mutation
origin
Mutant Cell Lines:  HEPD0589_5_B02, HEPD0589_5_D03, HEPD0589_5_F02, HEPD0636_3_A09, HEPD0636_3_A10, HEPD0636_3_B09, HEPD0636_3_B11, HEPD0636_3_C09, HEPD0636_3_C11, HEPD0636_3_D09, HEPD0636_3_D11, HEPD0636_3_E11, HEPD0636_3_F09, HEPD0636_3_F10, HEPD0636_3_G09, HEPD0636_3_G10, HEPD0636_3_G12, HEPD0636_3_H10
Germline Transmission:  Earliest citation of germline transmission: J:204812
Parent Cell Line:  JM8A3.N1 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Project Collection: EUCOMM
Mutation
description
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_Bact_P
 
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 95816164 of Chromosome 8 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 95816925. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 41 assay results
In Structures Affected by this Mutation: 8 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 1 line available
Carrying any Katnb1 Mutation:  36 strains or lines available
Notes
Cell line HEPD0636_3_F10 was successfully used to make chimeric mice. Germline transmission was accomplished. J:220901
References
Original:  J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015;
All:  5 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/19/2024
MGI 6.23
The Jackson Laboratory