Fads1tm1a(EUCOMM)Hmgu
Targeted Allele Detail
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Symbol: |
Fads1tm1a(EUCOMM)Hmgu |
Name: |
fatty acid desaturase 1; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH |
MGI ID: |
MGI:4435372 |
Gene: |
Fads1 Location: Chr19:10160252-10174241 bp, + strand Genetic Position: Chr19, 6.54 cM
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IMPC: |
Fads1 gene page |
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Mutant Cell Lines: |
HEPD0548_9_A07, HEPD0548_9_A08, HEPD0548_9_A11, HEPD0548_9_B07, HEPD0548_9_B08, HEPD0548_9_B11, HEPD0548_9_B12, HEPD0548_9_C07, HEPD0548_9_C08, HEPD0548_9_C12, HEPD0548_9_D08, HEPD0548_9_E10, HEPD0548_9_F10 |
Germline Transmission: |
Unknown
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Parent Cell Line: |
JM8.N4 (ES Cell)
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Strain of Origin: |
C57BL/6N
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Project Collection: |
EUCOMM
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Allele Type: |
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Targeted (Conditional ready, Null/knockout, Reporter) |
Mutation: |
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Insertion
Vector: L1L2_Bact_P
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Mutation details: The L1L2_Bact_P cassette was inserted at position 10161844 of Chromosome 19 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 10165013. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml
(J:157065)
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Original: |
J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015; |
All: |
1 reference(s) |
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