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Polr3ctm1a(EUCOMM)Wtsi
Targeted Allele Detail
Nomenclature
Symbol: Polr3ctm1a(EUCOMM)Wtsi
Name: polymerase (RNA) III (DNA directed) polypeptide C; targeted mutation 1a, Wellcome Trust Sanger Institute
MGI ID: MGI:4431544
Gene: Polr3c  Location: Chr3:96711520-96727498 bp, - strand  Genetic Position: Chr3, 41.96 cM, cytoband F2
Mutation
origin
Mutant Cell Lines:  EPD0458_4_A05, EPD0458_4_A08, EPD0458_4_B05, EPD0458_4_B06, EPD0458_4_B08, EPD0458_4_C05, EPD0458_4_C07, EPD0458_4_C08, EPD0458_4_D05, EPD0458_4_D07, EPD0458_4_D08, EPD0458_4_E06, EPD0458_4_E07, EPD0458_4_E08, EPD0458_4_F05, EPD0458_4_F07, EPD0458_4_G06, EPD0458_4_G08, EPD0458_4_H05, EPD0458_4_H06, EPD0458_4_H07, EPD0458_4_H08
Germline Transmission:  Unknown
Parent Cell Line:  JM8A3.N1 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Project Collection: EUCOMM
Mutation
description
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_Bact_P
 
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 96723112 of Chromosome 3 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 96722283. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 1 line available
Carrying any Polr3c Mutation:  21 strains or lines available
References
Original:  J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010;
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
05/26/2020
MGI 6.15
The Jackson Laboratory