Col1a1<tm3(tetO-Pou5f1,-Sox2,-Klf4,-Myc)Jae> Targeted Allele Detail MGI Mouse (MGI:4430598)

Col1a1^{tm3(tetO-Pou5f1,-Sox2,-Klf4,-Myc)Jae}
Targeted Allele Detail

Summary

Symbol:	Col1a1^{tm3(tetO-Pou5f1,-Sox2,-Klf4,-Myc)Jae}
Name:	collagen, type I, alpha 1; targeted mutation 3, Rudolf Jaenisch
MGI ID:	MGI:4430598
Synonyms:	Col1a1^4F2A, Col1a1 4F2A, Col1a1-4F2A, Col1a1^tetO-4F2A, Col1a1::tetO-4F2A
Gene:	Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
Alliance:	Col1a1^{tm3(tetO-Pou5f1,-Sox2,-Klf4,-Myc)Jae} page

Mutation
origin

Germline Transmission:	Earliest citation of germline transmission: J:157298
Parent Cell Line:	KH2 (ES Cell)
Strain of Origin:	(C57BL/6 x 129S4/SvJae)F1

Mutation
description

Allele Type:

Targeted (Inducible, Inserted expressed sequence, RMCE-ready)

Inducer:

doxycycline/tetracycline

Mutation:

Insertion

Col1a1^{tm3(tetO-Pou5f1,-Sox2,-Klf4,-Myc)Jae} expresses 4 genes

Mutation details: RMCE on KH2 cells inserted a cassette that contains a loxP-flanked pgkNEOpolyA cassette, the tetracycline responsive element (TRE or tetO), the single polycistronic expression cassette 4F2A, and SV40 intron plus polyA signal into the 3'UTR. The 4F2A cassette contains the four mouse reprogramming genes Oct4 (Pou5f1; POU domain, class 5, transcription factor 1), Sox2 (SRY-box containing gene 2), Klf4 (Kruppel-like factor 4 (gut)), and c-Myc (Myc; myelocytomatosis oncogene) separated by three different viral 2A oligopeptides that mediate ribosomal skipping (P2A [from porcine teschovirus-1], T2A [from insect Thosea asigna virus], and E2A [equine rhinitis A virus], respectively). (J:157298)

Phenotypes

View phenotypes and curated references for all genotypes (concatenated display).

Find Mice (IMSR)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available Cell Lines: 0 lines available
Carrying any Col1a1 Mutation:	160 strains or lines available

Notes

The allele was made in KH2 cells that carry Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} and Col1a1^{tm13(neo/hygro*)Jae} (J:159351). This allele was generated with Col1a1^{tm13(neo/hygro*)Jae}.

References

Original:	J:157298 Carey BW, et al., Single-gene transgenic mouse strains for reprogramming adult somatic cells. Nat Methods. 2010 Jan;7(1):56-9
All:	14 reference(s)

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 04/09/2024 MGI 6.23