Relatm1.2Gho
Targeted Allele Detail
|
|
| Symbol: |
Relatm1.2Gho |
| Name: |
v-rel reticuloendotheliosis viral oncogene homolog A (avian); targeted mutation 1.2, Sankar Ghosh |
| MGI ID: |
MGI:3794988 |
| Synonyms: |
p65-R274A, S276A(RAPA) |
| Gene: |
Rela Location: Chr19:5687511-5698158 bp, + strand Genetic Position: Chr19, 4.34 cM, cytoband B1-3
|
| Alliance: |
Relatm1.2Gho page
|
|
| Germline Transmission: |
Earliest citation of germline transmission:
J:134684
|
| Parent Cell Line: |
TC1/TC-1 (ES Cell)
|
| Strain of Origin: |
129S6/SvEvTac
|
|
| Allele Type: |
|
Targeted (Not Specified) |
| Mutations: |
|
Insertion, Nucleotide substitutions
|
| |
|
Mutation details: By homologous recombination in ES cells, endogenous DNA sequence for arginine 274 and serine 276 residues in exon 7 of Rela gene were replaced with codons for alanine at both locations followed by a neo cassette flanked by two loxP sites inserted into intron after Exon 7. A neo cassette was then deleted either in the recombined ES cells by transfection of cre-recombinase or in mice by crossing with splicer mice harboring a Cre transgene, leaving only single loxP sites. The presence of the mutation was confirmed by RT-PCR and DNA sequencing. Somewhat reduced amount mutant protein was translated.
(J:134684)
|
|
|
View phenotypes and curated references for all genotypes (concatenated display).
|
| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
|
| Carrying any Rela Mutation: |
24 strains or lines available
|
|
| Original: |
J:134684 Dong J, et al., Repression of gene expression by unphosphorylated NF-kappaB p65 through epigenetic mechanisms. Genes Dev. 2008 May 1;22(9):1159-73 |
| All: |
1 reference(s) |
|
Analysis Tools
