Gt(ROSA)26Sortm7(RNAi:Gsk3b)Rkuhn
Targeted Allele Detail
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Symbol: |
Gt(ROSA)26Sortm7(RNAi:Gsk3b)Rkuhn |
Name: |
gene trap ROSA 26, Philippe Soriano; targeted mutation 7, Ralf Kuhn |
MGI ID: |
MGI:3785845 |
Synonyms: |
sh-Gsk3b-flox |
Gene: |
Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
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Alliance: |
Gt(ROSA)26Sortm7(RNAi:Gsk3b)Rkuhn page
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Allele Type: |
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Targeted (Conditional ready, Knockdown, RMCE-ready) |
Mutation: |
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Insertion
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Gt(ROSA)26Sortm7(RNAi:Gsk3b)Rkuhn involves 1 genes/genome features (Gsk3b)
View all
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Mutation details: Recombinase mediated cassette exchange (RMCE) was used to generate an allele where upon expression of Cre recombinase a knockdown RNAi is generated to Gsk3b mRNA. Short hairpin RNA was designed with a loxP-flanked stop cassette in the loop portion of the RNAi between the sense and anti-sense sequences. The RNAi was put under the control of a human U6 promoter and the cassette was placed downstream of a splice acceptor and a frt-flanked neomycin resistance gene. RMCE was then performed on ES cells containing the Gt(ROSA)26tm1Rkuhn allele. Upon expression of cre recombinase, protein expression of Gsk3b was reduced by 84%.
(J:133340)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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Carrying any Gt(ROSA)26Sor Mutation: |
942 strains or lines available
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Original: |
J:133340 Steuber-Buchberger P, et al., Simultaneous Cre-mediated conditional knockdown of two genes in mice. Genesis. 2008 Mar;46(3):144-51 |
All: |
1 reference(s) |
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