Summary | Mutation origin | Mutation description | Find Mice (IMSR) | References

Symbol:	Gt(ROSA)26Sortm5(RNAi:Mapk1,Mapk3)Rkuhn
Name:	gene trap ROSA 26, Philippe Soriano; targeted mutation 5, Ralf Kuhn
MGI ID:	MGI:3785823
Synonyms:	shErk-dhflox
Gene:	Gt(ROSA)26Sor  Location: Chr6:113044389-113054205 bp, - strand  Genetic Position: Chr6, 52.73 cM
Alliance:	Gt(ROSA)26Sortm5(RNAi:Mapk1,Mapk3)Rkuhn page

Germline Transmission:	Earliest citation of germline transmission: J:133340
Parent Cell Line:	IDG3.2 (ES Cell)
Strain of Origin:	(C57BL/6J x 129S6/SvEvTac)F1

Allele Type:    Targeted (Conditional ready, Knockdown, RMCE-ready) Mutation:    Insertion   Gt(ROSA)26Sortm5(RNAi:Mapk1,Mapk3)Rkuhn involves 2 genes/genome features (Mapk3, Mapk1) View all   Mutation details: Recombinase mediated cassette exchange (RMCE) was used to generate an allele where upon expression of Cre recombinase knockdown RNAis are generated to both Mapk1 and Mapk3. Short hairpin RNAs were designed with a loxP-flanked stop cassette in the loop portion of the RNAi between the sense and anti-sense sequences. The RNAis were both put under the control of separate human U6 promoters and the entire cassette was placed downstream of a splice acceptor and a frt-flanked neomycin resistance gene. RMCE was then performed on ES cells containing the Gt(ROSA)26tm1Rkuhn allele. Upon expression of cre recombinase, protein expression of Mapk1 and Mapk3 were reduced by 17% and 89%, respectively. (J:133340)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:	942 strains or lines available

Original:	J:133340 Steuber-Buchberger P, et al., Simultaneous Cre-mediated conditional knockdown of two genes in mice. Genesis. 2008 Mar;46(3):144-51
All:	1 reference(s)