Summary | Mutation origin | Mutation description | Phenotypes | Find Mice (IMSR) | References

Symbol:	Gt(ROSA)26Sortm1Afst
Name:	gene trap ROSA 26, Philippe Soriano; targeted mutation 1, A Francis Stewart
MGI ID:	MGI:4367459
Synonyms:	Rosa26-rox-LacZ TG, Rosa-rox
Gene:	Gt(ROSA)26Sor  Location: Chr6:113044389-113054205 bp, - strand  Genetic Position: Chr6, 52.73 cM
Alliance:	Gt(ROSA)26Sortm1Afst page

Germline Transmission:	Earliest citation of germline transmission: J:154134
Parent Cell Line:	R1 (ES Cell)
Strain of Origin:	(129X1/SvJ x 129S1/Sv)F1-Kitl+

Allele Type:    Targeted (Reporter) Mutation:    Insertion   Mutation details: A targeting vector was designed to encode a sequence for the neomycin resistance gene with a polyadenylation signal (STOP sequence), flanked by rox sites (dre recombinase recognition sequences) and followed by the lacZ gene. This was targeted to the Gt(ROSA)26Sor locus in R1 ES cells. Following dre recombinase-mediated excision, beta-galactosidase expression can be detected in cells which underwent recombination. (J:154134)

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:	942 strains or lines available

Original:	J:154134 Anastassiadis K, et al., Dre recombinase, like Cre, is a highly efficient site-specific recombinase in E. coli, mammalian cells and mice. Dis Model Mech. 2009 Sep-Oct;2(9-10):508-15
All:	4 reference(s)