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Ikbkaptm1a(KOMP)Wtsi
Targeted Allele Detail
Nomenclature
Symbol: Ikbkaptm1a(KOMP)Wtsi
Name: inhibitor of kappa light polypeptide enhancer in B cells, kinase complex-associated protein; targeted mutation 1a, Wellcome Trust Sanger Institute
MGI ID: MGI:4362242
Gene: Ikbkap  Location: Chr4:56749680-56802331 bp, - strand  Genetic Position: Chr4, 31.66 cM
Mutation
origin
Mutant Cell Lines:  EPD0033_2_A09, EPD0033_2_A10, EPD0033_2_B09, EPD0033_2_B10, EPD0033_2_C09, EPD0033_2_C10, EPD0033_2_D09, EPD0033_2_D10, EPD0033_2_E10, EPD0033_2_F09, EPD0033_2_F10, EPD0033_2_G10, EPD0033_2_H09, EPD0033_2_H10
Germline Transmission:  Earliest citation of germline transmission: J:175295
Parent Cell Line:  JM8.F6 (ES Cell)
Strain of Origin:  C57BL/6N
Project Collection: KOMP-CSD
Mutation
description
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_gt0
 
Mutation detailsThe L1L2_gt0 cassette was inserted at position 56797052 of Chromosome 4 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 56796283. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other KOMP alleles can be found at http://www.knockoutmouse.org/aboutkompstrategies. (J:148605, J:173534)
Schematic for the Ikbkaptm1a(KOMP)Wtsi and Ikbkaptm1c(KOMP)Wtsi alleles
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 1 line available
Carrying any Ikbkap Mutation:  37 strains or lines available
Notes
Cell line EPD0033_2_A09 was successfully used to make chimeric mice. Germline transmission was accomplished by the IKMC (project 25621). J:202989
References
Original:  J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009;
All:  7 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
11/14/2017
MGI 6.11
The Jackson Laboratory