Summary | Mutation origin | Mutation description | Phenotypes | Find Mice (IMSR) | References

Symbol:	Pax1tm1(sb-pLHED)Jtak
Name:	paired box 1; targeted mutation 1, Junji Takeda
MGI ID:	MGI:4358156
Synonyms:	J0
Gene:	Pax1  Location: Chr2:147203850-147216972 bp, + strand  Genetic Position: Chr2, 72.63 cM
Alliance:	Pax1tm1(sb-pLHED)Jtak page

Germline Transmission:	Earliest citation of germline transmission: J:151776
Parent Cell Line:	v6.5 (ES Cell)
Strain of Origin:	(C57BL/6 x 129S4/SvJae)F1

Allele Type:    Targeted (Reporter) Mutation:    Insertion   Mutation details: A local hopping enhancer detector (LHED) construct was inserted into the 5' flank of the gene, over 49kb upstream of the translation start site. The LHED construct consisted of an frt site, a floxed Pgk1-neo cassette, a puromycin resistance gene, the sleeping beauty transposon pT2, a Pgk1 promoter, and a final frt site. The sleeping beauty transposon pT2 had been modified to contain a loxP site and the mouse Hspa1b minimal promoter fused to a lacZ gene. Removal of the transposon sequence results in expression of the puromycin resistance gene. Local hopping of the transposon allows for the creation of a nested series of deletion/inversion mutations via cre mediated recombination. (J:151776)

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Pax1 Mutation:	28 strains or lines available

Original:	J:151776 Kokubu C, et al., A transposon-based chromosomal engineering method to survey a large cis-regulatory landscape in mice. Nat Genet. 2009 Aug;41(8):946-52
All:	1 reference(s)