Gt(ROSA)26Sortm2(cre/ERT2)Brn
Targeted Allele Detail
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Symbol: |
Gt(ROSA)26Sortm2(cre/ERT2)Brn |
Name: |
gene trap ROSA 26, Philippe Soriano; targeted mutation 2, Anton Berns |
MGI ID: |
MGI:3764519 |
Synonyms: |
ERT2, Gt(ROSA)26Sortm2(cre/Esr1)Brn, Rosa26CreERT2, ROSA26-CreERT2 |
Gene: |
Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
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Alliance: |
Gt(ROSA)26Sortm2(cre/ERT2)Brn page
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Germline Transmission: |
Earliest citation of germline transmission:
J:127226
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Parent Cell Line: |
IB10/E14IB10 (ES Cell)
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Strain of Origin: |
129P2/OlaHsd
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Allele Type: |
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Targeted (Inducible, Recombinase) |
Inducer: |
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tamoxifen |
Mutation: |
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Insertion
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Gt(ROSA)26Sortm2(cre/ERT2)Brn expression driven by
1 gene
Knock-in expression driven by:
Organism |
Driver Gene |
Note |
mouse |
Gt(ROSA)26Sor (MGI:104735) |
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Mutation details: The endogenous locus was modified by insertion into the first intron of a construct containing the rabbit beta-globin 3' splice site followed by the cre/ERT2 fusion gene, which encodes Cre recombinase joined to a mutant hormone-binding domain of the human estrogen receptor that is activated by 4-hydroxytamoxifen (4-OHT), but not by the natural ER ligand 17-beta-estradiol. The fusion gene is followed by the rabbit beta-globin polyadenylation signal and a PGK-puro (puromycin resistance) cassette. Western blot analysis confirmed expression of the cre/ERT2 fusion product.
(J:127226)
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Activity: |
Tissue activity of this recombinase allele
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Driver:
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Gt(ROSA)26Sor
(mouse)
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View phenotypes and curated references for all genotypes (concatenated display).
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Original: |
J:127226 Hameyer D, et al., Toxicity of ligand-dependent Cre recombinases and generation of a conditional Cre deleter mouse allowing mosaic recombination in peripheral tissues. Physiol Genomics. 2007 Sep 19;31(1):32-41 |
All: |
102 reference(s) |
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