Summary | Mutation origin | Mutation description | Expression | Phenotypes | Disease models | Find Mice (IMSR) | References

Symbol: Il4ratm2Tch Name: interleukin 4 receptor, alpha; targeted mutation 2, Talal A Chatila MGI ID: MGI:3720593 Synonyms: IL-4RalphaR576, Il4raR576, Q576R Gene: Il4ra  Location: Chr7:125151443-125178646 bp, + strand  Genetic Position: Chr7, 68.94 cM Alliance: Il4ratm2Tch page

Lung histology staining of OVA-sensitized control (Q576) and Il4ratm2Tch/Il4ratm2Tch (R576) mice challenged intranasally with OVA on three consecutive days Show the 4 phenotype image(s) involving this allele.

Germline Transmission:	Earliest citation of germline transmission: J:152376
Parent Cell Line:	RW-4 (ES Cell)
Strain of Origin:	129X1/SvJ

Allele Type:    Targeted Mutation:    Insertion   Mutation details: The targeting vector was designed (by site-directed mutagenesis) to change the alanine-guanine to guanine-alanine at basepair 1964-65 of murine IL-4Ra cDNA, corresponding to a single amino acid substitution of glutamine to arginine at codon 576 (Q576R). This also inserted a loxp-flanked PGK-neo cassette within intron 11. The construct was electroporated into 129X1/SvJ-derived RW4 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a Cre-expressing vector to remove the selection cassette (leaving a single loxp site in intron 11) and then injected into C57BL/6 blastocysts. (J:152376)

Generation of the Il4ratm2Tch allele

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

3 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Il4ra Mutation:	41 strains or lines available

Original:	J:152376 Tachdjian R, et al., Pathogenicity of a disease-associated human IL-4 receptor allele in experimental asthma. J Exp Med. 2009 Sep 28;206(10):2191-204
All:	4 reference(s)