Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Apoetm2(APOE*3)Mae
Name:	apolipoprotein E; targeted mutation 2, Nobuyo Maeda
MGI ID:	MGI:2157240
Synonyms:	3, Apoe3, APOE3, APOE*3, apoE3-KI, apoE3 TR, Apoetm2(APOE)Mae, apoE TR, E3, TgHAPOE3N8, TgH/ApoeE3/N8, TRE3
Gene:	Apoe  Location: Chr7:19430034-19433113 bp, - strand  Genetic Position: Chr7, 9.94 cM
Alliance:	Apoetm2(APOE*3)Mae page

Germline Transmission:	Earliest citation of germline transmission: J:41705
Parent Cell Line:	E14TG2a (ES Cell)
Strain of Origin:	129P2/OlaHsd

Allele Type:    Targeted (Humanized sequence, Inserted expressed sequence, Modified isoform(s), Null/knockout) Mutations:    Insertion, Intragenic deletion   Apoetm2(APOE*3)Mae expresses 1 gene Knock-in expresses: Organism Expressed Gene Homolog in Mouse Note human APOE (348) Apoe  (MGI:88057) Expresses the epsilon3 isoform   Mutation details: A DNA fragment containing exons 2-4 of the human APOE gene (the APOE3 isoform) replaced an equivalent portion of the mouse Apoe gene. Western blot analysis on plasma derived from homozygous mice demonstrated that the human protein was expressed from this allele and the endogenous mouse protein was not detectable. (J:41705)

View phenotypes and curated references for all genotypes (concatenated display).

In Mice Carrying this Mutation:

2 RNA-Seq or microarray experiment(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Apoe Mutation:	145 strains or lines available

Original:	J:41705 Sullivan PM, et al., Targeted replacement of the mouse apolipoprotein E gene with the common human APOE3 allele enhances diet-induced hypercholesterolemia and atherosclerosis. J Biol Chem. 1997 Jul 18;272(29):17972-80
All:	163 reference(s)