Summary | Mutation origin | Mutation description | Expression | Phenotypes | Disease models | Tumor data | Find Mice (IMSR) | References

Symbol:	Braftm1Mmcm
Name:	Braf transforming gene; targeted mutation 1, Martin McMahon
MGI ID:	MGI:3711771
Synonyms:	BrafCA, BrafCA-V600E, BRAFF-V600E, BRAFV600Eca
Gene:	Braf  Location: Chr6:39580171-39702397 bp, - strand  Genetic Position: Chr6, 18.43 cM
Alliance:	Braftm1Mmcm page

Germline Transmission:	Earliest citation of germline transmission: J:121715
Parent Cell Line:	E14 (ES Cell)
Strain of Origin:	129P2/OlaHsd

Allele Type:    Targeted (Conditional ready, No functional change) Mutation:    Insertion   Mutation details: A targeting vector was designed to insert a loxP site and the human BRAF cDNA covering exons 15-18 into intron 14, along with a floxed neo cassette. A modified exon 15 was engineered into the vector to encode BrafV600E and a silent XbaI restriction site. RT-PCR confirmed that mutant transcript was expressed at the same level as wild-type, and that the V600E mutation was not detected. (J:121715)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

10 anatomical structure(s)

List all tumor models in MMHCdb carrying Braftm1Mmcm

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 3 strains available      Cell Lines: 0 lines available
Carrying any Braf Mutation:	58 strains or lines available

Original:	J:121715 Dankort D, et al., A new mouse model to explore the initiation, progression, and therapy of BRAFV600E-induced lung tumors. Genes Dev. 2007 Feb 15;21(4):379-84
All:	182 reference(s)