Summary | Transgene origin | Transgene description | Find Mice (IMSR) | References

Symbol:	TgTn(sb-T2/GT3/tTA)6600Dla
Name:	transgenic transposon concatemer 6600, David A Largaespada
MGI ID:	MGI:3698641
Synonyms:	T2/GT3/tTA
Transgene:	TgTn(sb-T2/GT3/tTA)6600Dla  Location: unknown  Genetic Position: Chr11, Syntenic
Alliance:	TgTn(sb-T2/GT3/tTA)6600Dla page

Strain of Origin:

FVB/N

Transgene Type:    Transgenic (Transposon concatemer) Mutation:    Insertion   Mutation details: A concatamer of sleeping beauty transposable elements each consisting of the improved inverted repeat direct repeat sequence specific for sleeping beauty transposase flanking a carp beta-actin splice acceptor site, stop signals in each of the 3 reading frames, the encaphalomyocarditis virus IRES, the Tet-Off sequence, and SV40 late polyadenylation signal. In addition a splice acceptor site from mouse Hprt and a V5 epitope tag were placed in reverse orientation. Finally loxP sites were placed inside of the inverted repeat sequences. These elements are able to trap genes independent of insertion orientation. In line 6600. 27 copies of the transposable element are integrated in chromosome 11 (B5-C). (J:117975)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available

Original:	J:117975 Geurts AM, et al., Conditional gene expression in the mouse using a Sleeping Beauty gene-trap transposon. BMC Biotechnol. 2006;6:30
All:	2 reference(s)