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Cd3etm1Mal
Targeted Allele Detail
Summary
Symbol: Cd3etm1Mal
Name: CD3 antigen, epsilon polypeptide; targeted mutation 1, Bernard Malissen
MGI ID: MGI:2155092
Synonyms: Cd3e-, Cd3-edelta5, Cd3-edelta55, CD3epsilon-, CD3-epsilondelta5, CD3epsilondelta5
Gene: Cd3e  Location: Chr9:44910038-44920925 bp, - strand  Genetic Position: Chr9, 24.84 cM
Alliance: Cd3etm1Mal page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:29341
Parent Cell Line:  Bruce 4 (ES Cell)
Strain of Origin:  B6.Cg-Thy1a
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsA PGK-neomycin cassette replaced most of exon 5, which encodes the extracellular domain. Northern analysis detected a faint, aberrant ~1.3kb transcript in thymocytes of homozygous mutant mice. PCR studies of cDNA synthesized from total thymocyte RNA did not detect wild-type mRNA in homozygous mutant mice using an internal, exon 8 oligo probe. However, these studies did detect a faint truncated transcript in homozygous mutant thymocytes, indicating that exon 5 was skipped in splicing events in the mutant allele. Western blot analysis of homozygous mutant thymocytes using a rabbit antiserum to the cytoplasmic carboxy terminal ten amino acids detected a small amount of a truncated protein. (J:29341)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Disease models
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Expression
In Mice Carrying this Mutation: 1 assay results
In Structures Affected by this Mutation: 1 anatomical structure(s)
Tumor Data
List all tumor models in MMHCdb carrying Cd3etm1Mal
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 4 strains available      Cell Lines: 0 lines available
Carrying any Cd3e Mutation:  47 strains or lines available
References
Original:  J:29341 Malissen M, et al., Altered T cell development in mice with a targeted mutation of the CD3-epsilon gene. EMBO J. 1995 Oct 2;14(19):4641-53
All:  120 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/19/2024
MGI 6.23
The Jackson Laboratory