Summary | Mutation origin | Mutation description | Expression | Phenotypes | Disease models | Find Mice (IMSR) | References

Symbol:	Cdk4tm1Bbd
Name:	cyclin dependent kinase 4; targeted mutation 1, Mariano Barbacid
MGI ID:	MGI:2154520
Synonyms:	Cdk4-, Cdk4n, Cdk4nc, Cdk4neo
Gene:	Cdk4  Location: Chr10:126899404-126903157 bp, + strand  Genetic Position: Chr10, 74.5 cM, cytoband D3
Alliance:	Cdk4tm1Bbd page

Germline Transmission:	Earliest citation of germline transmission: J:54534
Parent Cell Line:	R1 (ES Cell)
Strain of Origin:	(129X1/SvJ x 129S1/Sv)F1-Kitl+

Allele Type:    Targeted (Null/knockout) Mutations:    Insertion, Nucleotide substitutions   Mutation details: A loxP-flanked neomycin resistance cassette was inserted between exons 1 and 2. In addition, the AAGCCCGA sequence in exon 2 (nt 81-88) was replaced by AAGCTTGT. This alters codon 24 from one corresponding to arginine to a cysteine. A third silent substitution at position nt85 was used to create a diagnostic restriction enzyme site to monitor the presence of the mutation. RT-PCR analysis revealed that no transcript was produced from this allele in testis, ovaries or pancreas. Western blot analysis demonstrated that no protein was detectable in fibroblasts isolated from E14.5 homozygous embryos. (J:54534)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

11 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Cdk4 Mutation:	57 strains or lines available

Original:	J:54534 Rane SG, et al., Loss of Cdk4 expression causes insulin-deficient diabetes and Cdk4 activation results in beta-islet cell hyperplasia. Nat Genet. 1999 May;22(1):44-52
All:	20 reference(s)