Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Afptm1(Cebpa)Ndw
Name:	alpha fetoprotein; targeted mutation 1, Nai-dy Wang
MGI ID:	MGI:3610508
Gene:	Afp  Location: Chr5:90638596-90656766 bp, + strand  Genetic Position: Chr5, 44.77 cM
Alliance:	Afptm1(Cebpa)Ndw page

Germline Transmission:	Earliest citation of germline transmission: J:103404
Parent Cell Line:	AB2.2 (ES Cell)
Strain of Origin:	129S7/SvEvBrd-Hprt1b-m2

Allele Type:    Targeted (Inserted expressed sequence, Null/knockout) Mutation:    Insertion   Mutation details: Cebpa cDNA and a neomycin resistance gene in opposite orientation replaced exon 1 of the alpha fetoprotein locus. Quantitative RT-PCR of fetal livers demonstrated that mutants had a higher level of Cebpa mRNA than wild-type livers. It was undetermined whether those higher levels were due to expression of the knock-in gene alone or due to a combination of the knock-in gene plus elevated expression of the endogenous gene. (J:103404)

View phenotypes and curated references for all genotypes (concatenated display).

In Mice Carrying this Mutation:

40 assay results

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Afp Mutation:	32 strains or lines available

Original:	J:103404 Tan EH, et al., CCAAT/enhancer binding protein alpha knock-in mice exhibit early liver glycogen storage and reduced susceptibility to hepatocellular carcinoma. Cancer Res. 2005 Nov 15;65(22):10330-7
All:	1 reference(s)