Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Drd5tm1Sib
Name:	dopamine receptor D5; targeted mutation 1, David R Sibley
MGI ID:	MGI:3528074
Synonyms:	D5-
Gene:	Drd5  Location: Chr5:38476742-38479868 bp, + strand  Genetic Position: Chr5, 20.4 cM
Alliance:	Drd5tm1Sib page

Germline Transmission:	Earliest citation of germline transmission: J:95908
Parent Cell Line:	J1 (ES Cell)
Strain of Origin:	129S4/SvJae

Allele Type:    Targeted (Null/knockout) Mutation:    Insertion   Mutation details: A neo was inserted in reverse orientation to disrupt the reading frame within the coding region. A stop codon was engineered into the proximal neo linker to prematurely truncate the transcript subsequent to Gly-190 in the second extracellular loop. RT-PCR verified presence of the recombinant transcript in mutant brains. Immunohistochemistry using peptides from the region downstream of the stop codon showed that mutants did not produce the corresponding protein. (J:95908)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

5 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Drd5 Mutation:	39 strains or lines available

Original:	J:95908 Hollon TR, et al., Mice lacking D5 dopamine receptors have increased sympathetic tone and are hypertensive. J Neurosci. 2002 Dec 15;22(24):10801-10
All:	24 reference(s)