Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Phox2btm1(Phox2a)Mist
Name:	paired-like homeobox 2b; targeted mutation 1, Michele Studer
MGI ID:	MGI:3521828
Synonyms:	Phox2bKI2a
Gene:	Phox2b  Location: Chr5:67251740-67256469 bp, - strand  Genetic Position: Chr5, 35.95 cM
Alliance:	Phox2btm1(Phox2a)Mist page

Germline Transmission:	Earliest citation of germline transmission: J:126634
Parent Cell Line:	PC3 (ES Cell)
Strain of Origin:	129S4/SvJae-Tg(Prm-cre)70Og

Allele Type:    Targeted Mutation:    Insertion   Mutation details: Targeting took place in PC3 ES cells homozygous for Tg(Prm-cre)70Og, a cre transgene that expresseses Cre recombinase only in halpoid spermatids under control of the male-germline specific protamine promoter. The Phox2a coding sequence was inserted upstream of exon 1 along with an IRES-GFP and a floxed neo cassette, which was removed by cre-mediated recombination upon passage through the male germline. In situ hybridization and immunohistochemistry confirm the altered expression pattern of the knocked in coding region and absence of endogenous target gene expression. For unknown reasons the EGFP reporter was nonfunctional. (J:126634)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

9 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Phox2b Mutation:	25 strains or lines available

Original:	J:126634 Coppola E, et al., Reciprocal gene replacements reveal unique functions for Phox2 genes during neural differentiation. EMBO J. 2005 Dec 21;24(24):4392-403
All:	2 reference(s)