Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Sox10tm2(rtTA)Weg
Name:	SRY (sex determining region Y)-box 10; targeted mutation 2, Michael Wegner
MGI ID:	MGI:3513104
Synonyms:	Sox10rtTA
Gene:	Sox10  Location: Chr15:79039113-79048690 bp, - strand  Genetic Position: Chr15, 37.7 cM
Alliance:	Sox10tm2(rtTA)Weg page

Germline Transmission:	Earliest citation of germline transmission: J:94207
Parent Cell Line:	E14.1 (ES Cell)
Strain of Origin:	129P2/OlaHsd

Allele Type:    Targeted (Null/knockout, Transactivator) Mutation:    Insertion   Mutation details: Exons 3-5 (the entire coding region) were replaced with a reverse tetracycline controlled transactivator (rtTA) gene and a loxP-flanked Neo cassette, placing the start codon of the rtTA at the position of the endogenous start codon in exon 3, via homologous recombination. The Neo cassette was removed by crossing to mice expressing germline Cre-recombinase. Crossing to mice expressing lacZ under the control of the tetracycline inducible promoter and then treating with doxycycline resulted in X-gal staining (indicating rtTA activity) that recapitulated Sox10 expression. (J:94207)

View phenotypes and curated references for all genotypes (concatenated display).

In Structures Affected by this Mutation:

2 anatomical structure(s)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Sox10 Mutation:	33 strains or lines available

Original:	J:94207 Ludwig A, et al., Sox10-rtTA mouse line for tetracycline-inducible expression of transgenes in neural crest cells and oligodendrocytes. Genesis. 2004 Nov;40(3):171-5
All:	13 reference(s)