Summary | Mutation origin | Mutation description | Phenotypes | Find Mice (IMSR) | References

Symbol:	F9tm2(F9)Dws
Name:	coagulation factor IX; targeted mutation 2, Darrel W Stafford
MGI ID:	MGI:3055509
Synonyms:	R333Q-hFIX
Gene:	F9  Location: ChrX:59044824-59076119 bp, + strand  Genetic Position: ChrX, 33.5 cM, cytoband A6-A7
Alliance:	F9tm2(F9)Dws page

Germline Transmission:	Earliest citation of germline transmission: J:92964
Parent Cell Line:	TC1/TC-1 (ES Cell)
Strain of Origin:	129S6/SvEvTac

Allele Type:    Targeted Mutation:    Insertion   Mutation details: The targeting construct includes the signal sequence, first intron and propeptide of the mouse gene, the equivalent human cDNA and a neomycin cassette. Two site specific point mutations, T148A and R333Q were created in the human cDNA. The human cDNA is joined to the mouse gene at codons 6 and 7, such that the mouse expresses a missense mutant human protein, but does not express the mouse protein. ELISA analysis using specific human anti-F9 polyclonal antibodies detects human F9 in homozygous mice. (J:92964)

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any F9 Mutation:	8 strains or lines available

Original:	J:92964 Jin DY, et al., Creation of a mouse expressing defective human factor IX. Blood. 2004 Sep 15;104(6):1733-9
All:	1 reference(s)