Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Spi1tm1Kast
Name:	Spi-1 proto-oncogene; targeted mutation 1, Philippe Kastner
MGI ID:	MGI:3044765
Synonyms:	PU.1G, PU-1-GFP
Gene:	Spi1  Location: Chr2:90912750-90946104 bp, + strand  Genetic Position: Chr2, 50.44 cM, cytoband E3
Alliance:	Spi1tm1Kast page

Germline Transmission:	Earliest citation of germline transmission: J:90543
Parent Cell Line:	P1 (ES Cell)
Strain of Origin:	129S2/SvPas

Allele Type:    Targeted (Null/knockout, Reporter) Mutations:    Insertion, Intragenic deletion   Mutation details: A 22 bp segment of exon 1 was replaced with a rabbit beta globin intron, an enhanced GFP sequence and an SV40 polyadenylation signal via homologous recombination. Both mRNA and protein were undetectable in fetal liver from homozygous mice. GFP expression correlated well with endogenous Sfpi1 expression. (J:90543)

View phenotypes and curated references for all genotypes (concatenated display).

In Mice Carrying this Mutation:

14 assay results

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Spi1 Mutation:	27 strains or lines available

Original:	J:90543 Back J, et al., PU.1 determines the self-renewal capacity of erythroid progenitor cells. Blood. 2004 May 15;103(10):3615-23
All:	9 reference(s)