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Msh2tm1Wed
Targeted Allele Detail
Summary
Symbol: Msh2tm1Wed
Name: mutS homolog 2; targeted mutation 1, Winfried Edelmann
MGI ID: MGI:2680817
Synonyms: Msh2G674A, Msh2tm1Mtn
Gene: Msh2  Location: Chr17:87979960-88031141 bp, + strand  Genetic Position: Chr17, 57.87 cM
Alliance: Msh2tm1Wed page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:86278
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  Not Specified
Mutation
description
Allele Type:    Targeted (Inserted expressed sequence)
Mutation:    Insertion
 
Mutation detailsThe incorporation of an engineered point mutation in exon 13 created a glycine to alanine substitution at codon 674 (G674A), within the conserved ATPase domain of the C-terminal. A floxed neo-PGK-hygro cassette that was included in the targeting vector was removed by cre-mediated recombination leaving a single loxP site and the G674A mutation. Western blot analysis of homozygous mutant MEFs indicated expression of the mutant protein at wild-type levels. (J:88092)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Msh2 Mutation:  95 strains or lines available
References
Original:  J:86278 Martin A, et al., Msh2 ATPase activity is essential for somatic hypermutation at a-T basepairs and for efficient class switch recombination. J Exp Med. 2003 Oct 20;198(8):1171-8
All:  4 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/19/2024
MGI 6.23
The Jackson Laboratory