Mapkapk5tm1Mgl
Targeted Allele Detail
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| Symbol: |
Mapkapk5tm1Mgl |
| Name: |
MAP kinase-activated protein kinase 5; targeted mutation 1, Matthias Gaestel |
| MGI ID: |
MGI:2680287 |
| Synonyms: |
deltaex6, MK5- |
| Gene: |
Mapkapk5 Location: Chr5:121663114-121683955 bp, - strand Genetic Position: Chr5, 61.84 cM, cytoband F
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| Alliance: |
Mapkapk5tm1Mgl page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:86268
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| Parent Cell Line: |
E14.1 (ES Cell)
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| Strain of Origin: |
129P2/OlaHsd
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| Allele Type: |
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Targeted (Null/knockout) |
| Mutations: |
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Insertion, Intragenic deletion
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Mutation details: Exon 6 was replaced with a neo cassette. The targeted exon encoded amino acids 131 through 161 which make up portions of the kinase catalytic core subdomains VIa and VIb. Northern blot and RT-PCR analyses identified mutant transcript approximately 100 bp shorter than normal transcript. Sequence analysis showed the mutant transcript to contain neither exon 6 nor the neo cassette. Low levels of truncated protein were detected by Western blot analysis, but this protein was reported to lack catalytic activity.
(J:86268)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Mapkapk5 Mutation: |
30 strains or lines available
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| Original: |
J:86268 Shi Y, et al., Elimination of protein kinase MK5/PRAK activity by targeted homologous recombination. Mol Cell Biol. 2003 Nov;23(21):7732-41 |
| All: |
5 reference(s) |
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Analysis Tools
