Summary | Mutation origin | Mutation description | Expression | Phenotypes | Find Mice (IMSR) | References

Symbol:	Wt1tm1Hst
Name:	WT1 transcription factor; targeted mutation 1, Nicholas D Hastie
MGI ID:	MGI:2654351
Gene:	Wt1  Location: Chr2:104956874-105003959 bp, + strand  Genetic Position: Chr2, 55.06 cM, cytoband E
Alliance:	Wt1tm1Hst page

Germline Transmission:	Earliest citation of germline transmission: J:82515
Parent Cell Line:	E14 (ES Cell)
Strain of Origin:	129P2/OlaHsd

Allele Type:    Targeted (Modified isoform(s), Null/knockout) Mutations:    Insertion, Single point mutation   Mutation details: A nonsense mutation was introduced into exon 1, 12 codons downstream of the CUG start codon and upstream of the AUG start codon. A floxed hygro cassette was excised in vitro, leaving a single loxP site in a nonconserved region within in intron 1. Western blot analysis of homozygous mutant fetal kidney and testis tissue showed that while the CUG-initiated isoforms were undetected, normal levels of AUG-initiated isoforms were present. (J:82515)

View phenotypes and curated references for all genotypes (concatenated display).

In Mice Carrying this Mutation:

3 assay results

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Wt1 Mutation:	34 strains or lines available

Original:	J:82515 Miles CG, et al., Mice lacking the 68-amino-acid, mammal-specific N-terminal extension of WT1 develop normally and are fertile. Mol Cell Biol. 2003 Apr;23(7):2608-13
All:	2 reference(s)