Summary | Mutation origin | Mutation description | Phenotypes | Find Mice (IMSR) | References

Symbol:	Cd247tm2Tks
Name:	CD247 antigen; targeted mutation 2, Takashi Saito
MGI ID:	MGI:2446976
Synonyms:	Cd247/Pou2f1tm1Tks, Cd247tm1Tks, Cd3z/Pou2f1tm1Tks, etaT, Pou2f1tm1Tks
Gene:	Cd247  Location: Chr1:165616250-165704846 bp, + strand  Genetic Position: Chr1, 73.14 cM, cytoband G-H
Alliance:	Cd247tm2Tks page

Germline Transmission:	Earliest citation of germline transmission: J:17251
Parent Cell Line:	E14.1 (ES Cell)
Strain of Origin:	129P2/OlaHsd

Allele Type:    Targeted (Null/knockout) Mutations:    Insertion, Intergenic deletion   Cd247tm2Tks involves 1 genes/genome features (Pou2f1) View all   Mutation details: The replacement of a 1.4 kb genomic fragment with a neo cassette resulted in the disruption of both the Cd247 and Pou2f1 loci, which are located at the same chromosomal position on opposite strands. The deletion of a 3' portion of Cd247 exon 9 and its splice acceptor site disrupted sequence encoding the eta isoform while leaving the sequence encoding the zeta isoform intact. Neither eta transcript nor protein was detected in homozygous mutant mice while normal expression of zeta chain homodimers was confirmed by 2D SDS-PAGE analysis. The insertion of the neo cassette also replaced the 3' UTR of the Pou2f1 locus, resulting in reduced expression of normal transcript as well as in an induced expression of aberrant transcript. (J:17251)

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Cd247 Mutation:	11 strains or lines available

Original:	J:17251 Ohno H, et al., Targeted disruption of the CD3 eta locus causes high lethality in mice: modulation of Oct-1 transcription on the opposite strand. EMBO J. 1994 Mar 1;13(5):1157-65
All:	1 reference(s)