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Phenotypes Associated with This Genotype
Genotype
MGI:5811565
Allelic
Composition
Coro7tm1a(EUCOMM)Hmgu/Coro7tm1a(EUCOMM)Hmgu
Genetic
Background
C57BL/6N-Coro7tm1a(EUCOMM)Hmgu
Cell Lines HEPD0658_3_B04
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Coro7tm1a(EUCOMM)Hmgu mutation (0 available); any Coro7 mutation (3 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• primary dermal fibroblasts isolated from mutant neonates exhibit severe dispersal of the Golgi ribbon from the perinuclear position
• 38% of mutant fibroblasts show fragmented Golgi relative to 12% wild-type cells
• only 34% of mutant fibroblasts have the Golgi ribbon compact and lying within tens of micrometres from the nucleus relative to ~62% in wild-type cells
• scratch-wounded mutant fibroblasts in the first row reorganize their Golgi apparatus and reposition the centrosome (microtubule organizing center, MTOC) towards the leading edge faster than wild-type fibroblasts
• at 3 h post-wounding, the % of reoriented (polarized) Golgi and MTOC is significantly increased to 81% and 75% relative to 66% and 58%, respectively, in wild-type cells; however, no differences are noted at 7 h after wounding
• mutant fibroblasts show prominent actin filaments traversing the scattered Golgi stacks, suggesting that F-actin is increased at the Golgi apparatus
• in a scratch-wound assay, 65% of mutant fibroblasts have reoriented their actin cytoskeleton perpendicularly to the wound edge at 3 h post-wounding relative to only 34% in wild-type cells where most actin fibers remain oriented parallel to the wound
• mutant fibroblasts exhibit massive F-actin filaments and a 1.5-fold increase in F-actin content relative to wild-type cells
• mutant fibroblasts adhere more than wild-type cells and attach firmly to a fibronectin (FN)-coated surface within 30 min of seeding
• cell spreading and focal adhesions are significantly increased within 1 h of plating relative to wild-type cells
• in a scratch-wound assay, mutant fibroblasts exhibit a significantly higher mean migration speed than wild-type cells (15 um/h versus 8 um/h), leading to a faster gap closure after 20 h of wounding

homeostasis/metabolism
• in a scratch-wound assay, mutant fibroblasts exhibit a significantly higher migration speed than wild-type cells, leading to a faster gap closure after 20 h of wounding
• however, the % of proliferating cells is normal, as shown by Ki-67 staining


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
08/13/2019
MGI 6.14
The Jackson Laboratory