Phenotypes Associated with This Genotype

Genotype
MGI:5796492

• Allelic Composition: Tg(Pten)1Srn/0
• Genetic Background: involves: C57BL/6 * CBA

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

extended life span ( J:182671 )

• the mean longevity of the oldest 20% of mice is increased by 16% in males and 9% in females and the mean longevity of the oldest 10% of mice is also increased

postnatal lethality, incomplete penetrance ( J:182671 )

• early postnatal lethality, with frequency of weaned mice about 50% lower than expected

growth/size/body

decreased body fat mass ( J:182671 )

• mice show a relative decrease in fat mass

decreased embryo size ( J:182671 )

• E13.5 embryos are smaller

decreased body weight ( J:182671 )

• 27% and 28% decrease in weight of 4-6 month old males and females, respectively

• 35% and 44% decrease in weight of 1-2 year old males and females, respectively

nervous system

abnormal excitatory postsynaptic potential ( J:234430 )

• the slope of the synaptically evoked field potential is decreased in acute hippocampal slices

• no further depression is seen following synthetic amyloid beta incubation as seen in wild-type hippocampal slices

adipose tissue

abnormal adipose tissue morphology ( J:182671 )

• subcutaneous (inguinal) white adipose tissue and visceral (epididymal) white adipose tissue shows the presence of abundant brown adipocytes interspersed within white adipocytes

decreased body fat mass ( J:182671 )

• mice show a relative decrease in fat mass

abnormal brown adipose tissue morphology ( J:182671 )

• the interscapular brown adipose tissue shows a more intense color and a denser appearance due to reduced size of the multilocular lipid droplets

decreased brown fat cell lipid droplet size ( J:182671 )

• reduction in size of multilocular lipid droplets in the interscapular brown adipose tissue

decreased white fat cell size ( J:182671 )

♂ • size of adipocytes in the epididymal white adipose tissue is smaller

decreased epididymal fat pad weight ( J:182671 )

♂ • weight of the epididymal white adipose tissue relative to body weight is lower in males

increased adipocyte glucose uptake ( J:182671 )

• uptake of glucose is higher in the brown adipose tissue of mutants compared to wild-type mice

abnormal brown adipose tissue physiology ( J:182671 )

• mice exhibit hyperactive brown adipose tissue

behavior/neurological

polyphagia ( J:182671 )

enhanced coordination ( J:182671 )

• 1.5-2 year old mice perform better in the tightrope test

cellular

abnormal cell physiology ( J:182671 )

• MEFs are more resistant than wild-type MEFs to oncogenic transformation

• marker analysis indicates a lower level of DNA damage in the liver of old mice compared to wild-type mice

increased adipocyte glucose uptake ( J:182671 )

• uptake of glucose is higher in the brown adipose tissue of mutants compared to wild-type mice

decreased cell proliferation ( J:182671 )

• mouse embryonic fibroblasts (MEFs) exhibit a poorer proliferative capacity compared to wild-type cells

embryo

decreased embryo size ( J:182671 )

• E13.5 embryos are smaller

homeostasis/metabolism

decreased fasting circulating glucose level ( J:182671 )

• both young (4-6 months) and old (1.5-2 years) mice exhibit lower fasting levels of glucose

decreased circulating insulin level ( J:182671 )

• both young and old mice exhibit lower insulin serum levels

decreased circulating insulin-like growth factor I level ( J:182671 )

• serum levels of Igf1 are moderately lower in both young and old mice

decreased circulating leptin level ( J:182671 )

• serum levels of leptin are reduced

• however, adiponectin and thyroxine levels are normal

decreased circulating cholesterol level ( J:182671 )

increased energy expenditure ( J:182671 )

• mice exhibit increased energy expenditure relative to their total body weight and under normal housing conditions

increased insulin sensitivity ( J:182671 )

• both young and old mice exhibit a lower value on the insulin resistance index

• mice fed a high-fat diet for 6 months respond to insulin injection with a significant decrease in glucose levels compared to wild-type mice which are unresponsive to insulin

increased basal metabolism ( J:182671 )

• mice exhibit increased resting metabolic rate relative to lean mass, both at 23 and 30 degrees Celcius

• however, locomotor activity, body temperature and respiratory quotient are normal

decreased incidence of tumors by chemical induction ( J:182671 )

• mice intramuscularly injected with 3-methyl-cholanthrene to induce fibrosarcomas show extended tumor-free survival compared to wild-type mice and a reduction in several cancer types, notably lymphomas and histiocytic sarcomas

liver/biliary system

decreased susceptibility to diet-induced hepatic steatosis ( J:182671 )

• high-fat diet fed mice are protected from liver steatosis

• however, high-fat diet fed mice increase body weight similarly to wild-type mice

neoplasm

decreased incidence of tumors by chemical induction ( J:182671 )

• mice intramuscularly injected with 3-methyl-cholanthrene to induce fibrosarcomas show extended tumor-free survival compared to wild-type mice and a reduction in several cancer types, notably lymphomas and histiocytic sarcomas