Phenotypes Associated with This Genotype

Genotype
MGI:5697051

• Allelic Composition: Usp28^{Gt(EUCE0037g10)Hmgu}/Usp28^{Gt(EUCE0037g10)Hmgu}
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

immune system phenotype ( J:215354 )

N • homozygotes show normal total thymocyte numbers and a normal profile of CD4 and CD8 single-positive and double-positive T lymphocytes relative to wild-type controls; TCR V(D)J joining events are normal with no detectable trans-rearrangements

N • B-cell proliferation and class switch recombination to IgG1 are normal following treatment with LPS or IL-4

N • serum levels of IgG1, IgG2B, and IgE are normal following inoculation with TNP-KLH

N • following whole-body irradiation, IR-induced apoptosis in the spleen and thymus is comparable to that in wild-type controls

N • ex vivo analysis revealed normal levels of thymocyte apoptosis after exposure to a range of IR doses

decreased spleen weight ( J:215354 )

• homozygotes display a slightly decreased spleen weight relative to wild-type controls

decreased splenocyte number ( J:215354 )

• homozygotes display slightly smaller spleens with reduced cellularity

cellular

cellular phenotype ( J:215354 )

N • following ionizing radiation (IR) or UV-C light treatment, mutant primary MEFs exhibit normal DNA damage-signaling relative to wild-type MEFs

N • following IR treatment, mutant MEFs show normal G2/M checkpoint arrest and recovery relative to wild-type MEFs

N • following IR or UV-C treatment, clonogenic survival curves are identical to those in wild-type MEFs

induced chromosome breakage ( J:215354 )

• in response to acute aphidicolin exposure, mutant MEFs show slightly increased levels of chromosomal aberrations relative to wild-type MEFs

hematopoietic system

decreased spleen weight ( J:215354 )

• homozygotes display a slightly decreased spleen weight relative to wild-type controls

decreased splenocyte number ( J:215354 )

• homozygotes display slightly smaller spleens with reduced cellularity

reproductive system

reproductive system phenotype ( J:215354 )

N • contrary to expectation, homozygotes are fertile

neoplasm

neoplasm ( J:215354 )

N • contrary to expectation, homozygotes do not exhibit increased tumor predisposition relative to wild-type controls