Phenotypes Associated with This Genotype

Genotype
MGI:5550554

• Allelic Composition: Nme8^tm1.1Arte/Nme8^tm1.1Arte; Txndc2^tm1.1Arte/Txndc2^tm1.1Arte
• Genetic Background: involves: C57BL/6 * C57BL/6NTac

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

mortality/aging ( J:205172 )

N • normal preweaning mortality rate comparing to wild-type

cellular

asthenozoospermia ( J:205172 )

• motility is normal at 6 months of age but total motility is reducedby 15% comparing to wild-type in 12 month old animals

• reduced motility is not associated with loss of cell viability

• progressive motility decreases by 30% at 18 months and 50% at 24 months of age comparing to wild-type controls

• only 10% of spermatozoa from 24 month old animals display forward, progressive motility

abnormal DNA repair ( J:205172 )

• increase in DNA damage in spermatozoa at both 12 and 18 months of age

oxidative stress ( J:205172 )

• increased 4HNE adducts in spermatozoa from two-year old animals

• spermatozoa at 18 months of age display high levels of reactive oxygen species

reproductive system

reproductive system phenotype ( J:205172 )

N • normal pregnancy rates, average number of pups per litter, and male-to-female pups ratio

N • normal sperm count, morphology, and mitochondrial membrane potential at 18 and 24 months old animals

abnormal sperm physiology ( J:205172 )

• spermatozoa at 18 months of age display high levels of reactive oxygen species

• however, no change in mitochondrial membrane potential is detectedin sperm from mice at 12 to 18 months of age

• levels of lipid peroxidation and DNA damage are increased in spermatozoa at 2 years and 6, 12, and 18 months of age, respectively

asthenozoospermia ( J:205172 )

• motility is normal at 6 months of age but total motility is reducedby 15% comparing to wild-type in 12 month old animals

• reduced motility is not associated with loss of cell viability

• progressive motility decreases by 30% at 18 months and 50% at 24 months of age comparing to wild-type controls

• only 10% of spermatozoa from 24 month old animals display forward, progressive motility

endocrine/exocrine glands

endocrine/exocrine gland phenotype ( J:205172 )

N • testes and caudal epididymal tissue sections show no abnormality comparing to wild-type

homeostasis/metabolism

abnormal DNA repair ( J:205172 )

• increase in DNA damage in spermatozoa at both 12 and 18 months of age

increased peroxidase activity ( J:205172 )

• increased level of lipid peroxidation in spermatozoa from two-year old animals