Analysis Tools
reproductive system
 |
• male fertility is blocked
• germ line transmission not achieved
|
cellular
|
N |
• mutant cells are morphologically normal
• apoptosis is not induced
|
endocrine/exocrine glands
|
|
• Rathke's pouch fails to detach from the oral ectoderm when the mutant cells comprise 75% or more of the chimera
|
|
|
• infundibulum becomes progressively more shallow as the percentage of mutant cells increases
• infundibulum is normal when the chimera is 5% mutant cells
• infundibulum is shallow when the chimera is 20% mutant cells
|
|
|
• infundibulum is absent when the chimera is 75% mutant cells
|
embryo
|
N |
• gastrulation is abnormal in only about 19% of chimeras (approximately normal percentage) as compared to 52% abnormal in knockout mice
|
nervous system
|
|
• Rathke's pouch fails to detach from the oral ectoderm when the mutant cells comprise 75% or more of the chimera
|
|
|
• infundibulum becomes progressively more shallow as the percentage of mutant cells increases
• infundibulum is normal when the chimera is 5% mutant cells
• infundibulum is shallow when the chimera is 20% mutant cells
|
|
|
• infundibulum is absent when the chimera is 75% mutant cells
|
