Phenotypes Associated with This Genotype

Genotype
MGI:5473703

• Allelic Composition: Cfl1^c5/Cfl1^c5; Vangl2^Lp/Vangl2^Lp
• Genetic Background: involves: C57BL/6J * LPT/LeJ

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:194042 )

• arrest at approximately E9.5

embryo

abnormal embryo turning ( J:194042 )

• fail to undergo turning

abnormal left-right axis patterning ( J:194042 )

• at E8.0 - E8.5 expression analysis indicates randomized left-right axis patterning

embryonic growth arrest ( J:194042 )

• at approximately E9.5

decreased embryo size ( J:194042 )

• shorter at E9.5

open neural tube ( J:194042 )

• open along the entire axis of the embryo at E9.5

abnormal notochord morphology ( J:194042 )

• midline is shorter and wider at the 1 to 4 somite stage compared to either single mutant

• threefold shorter and wider compared to wild-type controls, twofold shorter and wider compared to Vangl2^Lp single mutants

• change in size is not due to changes in cell shape

abnormal primitive node morphology ( J:194042 )

• expression analysis indicates many node cells lack posterior polarization

abnormal embryonic cilium location or orientation ( J:194042 )

• cilia are normal in length but do not point uniformly to the posterior and their position on the cell is more variable than in controls

abnormal somite development ( J:194042 )

abnormal somite size ( J:194042 )

• significantly reduced length to width ratio compared to either single mutant

cardiovascular system

abnormal heart looping ( J:194042 )

abnormal direction of heart looping ( J:194042 )

• in 2 of 6 embryos at E9.5

failure of heart looping ( J:194042 )

• a linear heart tube is seen at E9.5 in 1 of 6 embryos

growth/size/body

decreased embryo size ( J:194042 )

• shorter at E9.5

heterotaxia ( J:194042 )

• at E8.0 - E8.5 expression analysis indicates randomized left-right axis patterning

nervous system

open neural tube ( J:194042 )

• open along the entire axis of the embryo at E9.5

cellular

abnormal embryonic cilium location or orientation ( J:194042 )

• cilia are normal in length but do not point uniformly to the posterior and their position on the cell is more variable than in controls