Analysis Tools
limbs/digits/tail
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• at E16.5 in contrast to wild-type forelimbs where the spinodeltoidus (Spd), musculus triceps brachii longus (Tbl), and m triceps brachii lateralis (Tblt) muscles have specific origin and insertion sites, muscle bundles in the equivalent region of mutant limbs show a common origin at one focus and have split to form smaller muscle bundles that insert at aberrant sites when tamoxifen treatment is performed at E10.5; mispatterning of emerging muscles and ectopic splitting of nascent muscle bundles in observed by E13.5 with tamoxifen treatment at E10.5
• with tamoxifen treatment at E10.5, in the forelimb the m triceps brachii medialis (Tbm), Tbl, and Tblt, which insert on the olecranon process of the ulna in wild-type, show ectopic splitting into additional bundles which insert in the more distal shaft of the ulna
• zeugopodal muscles such as the m extensor digitorum communis (Edc) are ectopically split
• all limb musculature is affected
• limb muscle formation is unaffected if tamoxifen administration is done at E11.5
• terminal differentiation of limb muscle is not affected
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