Phenotypes Associated with This Genotype

Genotype
MGI:4436723

• Allelic Composition: Fgf21^tm1Nit/Fgf21^tm1Nit
• Genetic Background: C57BL/6-Fgf21^tm1Nit

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:157334 )

N • at 2 months of age, normally-fed male homozygotes display normal oxygen consumption and energy expenditure relative to wild-type controls

N • at 2 months of age, normally-fed male homozygotes show normal blood glucose, triglyceride, insulin and glucagon levels

N • at 2 months of age, both normally-fed and fasted male homozygotes show no significant differences in hepatic triglyceride or glycogen levels relative to similarly treated wild-type controls

increased circulating ketone body level ( J:157334 )

• the fasting induced increase in circulating levels beta-hydroxybutyrate is greater than that in fasted wild-type controls

• however, hepatic expression of ketogenic genes is similarly increased in fasted wild-type and mutant mice relative to that in normally-fed controls

abnormal circulating free fatty acids level ( J:157334 )

decreased circulating free fatty acids level ( J:157334 )

• a trend towards reduced levels of non-esterified fatty acids in blood when on a normal diet

increased circulating free fatty acids level ( J:157334 )

• non-esterified fatty acids in blood become elevated relative to controls when fasted

enhanced lipolysis ( J:157334 )

• lipolytic activity in the white adipose tissue is increased in fasted mice

• however, lipase gene (Lipe and Pnpla2) expression in subcutaneous white adipose tissue is significantly increased to similar levels in fasted wild-type and mutant mice

impaired lipolysis ( J:157334 )

• a trend towards reduced lipolytic activity in adipocytes

• at 2 months of age, normally-fed male homozygotes show a significant reduction in lipase gene (Lipe and Pnpla2) expression in subcutaneous white adipose tissue relative to wild-type controls

growth/size/body

increased body weight ( J:157334 )

• males display a trend towards being slightly heavier than controls at 8 weeks of age

increased liver weight ( J:157334 )

• liver weight is slightly and significantly heavier than controls at 2 months of age

liver/biliary system

increased liver weight ( J:157334 )

• liver weight is slightly and significantly heavier than controls at 2 months of age

adipose tissue

decreased white adipose tissue amount ( J:157334 )

• white adipose tissue weight displays a trend towards a decrease with fasting, unlike controls

increased white adipose tissue amount ( J:157334 )

• display a trend towards increased subcutaneous fat weight

• display a trend towards increased epididymal fat weight

abnormal white fat cell size ( J:157334 )

decreased white fat cell size ( J:157334 )

• adipocyte size is suggestive of a decrease with fasting

increased white fat cell size ( J:157334 )

• mature adipocytes display a trend towards larger size in subcutaneous and epididymal fat

behavior/neurological

behavior/neurological phenotype ( J:157334 )

N • at 2 months of age, male homozygotes show no differences in food intake or diurnal variation in food intake relative to wild-type controls