Phenotypes Associated with This Genotype

Genotype
MGI:3849479

• Allelic Composition: Gpr68^tm1.1Yaxu/Gpr68^tm1.1Yaxu
• Genetic Background: involves: 129S4/SvJae * C57BL/6 * FVB/N

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Brown adipose tissue abnormality in Gpr68^tm1.1Yaxu/Gpr68^tm1.1Yaxu mice compared to Gpr68^tm1Yaxu/Gpr68^tm1Yaxu mice

immune system

abnormal osteoclast differentiation ( J:149315 )

• fewer osteoclasts form in vitro from bone marrow cells than from Gpr68^tm1Yaxu cells

decreased macrophage cell number ( J:149315 )

• Background Sensitivity: unlike on a congenic C57BL/6 background, thioglycolate-treated mice produce fewer peritoneal macrophages than in similarly treated Gpr68^tm1Yaxu homozygotes

• however, macrophage production induced by macrophage colony-stimulating factor (M-CSF) is normal

increased monocyte cell number ( J:149315 )

• Background Sensitivity: unlike on a congenic C57BL/6 background, monocyte numbers are increased compared to in Gpr68^tm1Yaxu homozygotes

abnormal macrophage physiology ( J:149315 )

• LPS-induced nitric oxide (NO) production is less than in similarly treated Gpr68^tm1Yaxu homozygotes

abnormal osteoclast physiology ( J:149315 )

• osteoclast survival at pH 6.8 is more than that of osteoclasts from Gpr68^tm1Yaxu homozygotes

adipose tissue

abnormal brown adipose tissue morphology ( J:149315 )

• Background Sensitivity: unlike on a congenic C57BL/6 background, brown adipose tissue is less dense compared to in Gpr68^tm1Yaxu homozygotes

increased brown adipose tissue amount ( J:149315 )

• Background Sensitivity: unlike on a congenic C57BL/6 background, intrascapular brown adipose tissue deposit exhibit increased weight and size compared to in Gpr68^tm1Yaxu homozygotes

neoplasm

decreased tumor growth/size ( J:149315 )

• B16-F10 malignant cells form smaller tumors with reduced blood vessels than tumors that form when B16-F10 malignant cells are transplanted into Gpr68^tm1Yaxu homozygotes

skeleton

skeleton phenotype ( J:149315 )

N • bone volume/total volume, osteoblasts number, osteoclasts number, trabecular thickness, and bone marrow cavity are normal

abnormal osteoclast differentiation ( J:149315 )

• fewer osteoclasts form in vitro from bone marrow cells than from Gpr68^tm1Yaxu cells

abnormal osteoclast physiology ( J:149315 )

• osteoclast survival at pH 6.8 is more than that of osteoclasts from Gpr68^tm1Yaxu homozygotes

hematopoietic system

abnormal osteoclast differentiation ( J:149315 )

• fewer osteoclasts form in vitro from bone marrow cells than from Gpr68^tm1Yaxu cells

decreased macrophage cell number ( J:149315 )

• Background Sensitivity: unlike on a congenic C57BL/6 background, thioglycolate-treated mice produce fewer peritoneal macrophages than in similarly treated Gpr68^tm1Yaxu homozygotes

• however, macrophage production induced by macrophage colony-stimulating factor (M-CSF) is normal

increased monocyte cell number ( J:149315 )

• Background Sensitivity: unlike on a congenic C57BL/6 background, monocyte numbers are increased compared to in Gpr68^tm1Yaxu homozygotes

abnormal macrophage physiology ( J:149315 )

• LPS-induced nitric oxide (NO) production is less than in similarly treated Gpr68^tm1Yaxu homozygotes

abnormal osteoclast physiology ( J:149315 )

• osteoclast survival at pH 6.8 is more than that of osteoclasts from Gpr68^tm1Yaxu homozygotes

cellular

abnormal osteoclast differentiation ( J:149315 )

• fewer osteoclasts form in vitro from bone marrow cells than from Gpr68^tm1Yaxu cells