Phenotypes Associated with This Genotype

Genotype
MGI:3834654

• Allelic Composition: Tcra^Ln3Jae/Tcra⁺; Tcrb^Ln3Jae/Tcrb⁺
• Genetic Background: B6.Cg-Tcrb^Ln3Jae Tcra^Ln3Jae

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

thymus hypoplasia ( J:143006 )

• cellularity of the thymus is reduced by about 4-fold

abnormal T cell differentiation ( J:143006 )

• the pre-arranged TCR genes are expressed abnormally early with intracellular TCR-beta chains detectable in the 75% of the common lymphoid progenitors in the bone marrow

• these TCR-beta chains were also detectable in 35% of multipotent progenitors, 12% of short-term hemopoietic stem cells, and 5% of long-term hemopoietic stem cells of the bone marrow

abnormal double-negative T cell morphology ( J:143006 )

• there is a severe reduction in the percentage of thymocytes at the DN1-3 stages

• however, there is a large population of unusual double-negative thymocytes that are CD3⁺

• this double-negative CD3⁺ population is rarely found in the spleen

decreased double-positive T cell number ( J:143006 )

• there is a greater than 4-fold reduction in the percentage of double-positive thymocytes

• the double-positive thymus makes up 83% of the normal thymus but only 16% in these mutants

decreased CD4-positive, alpha-beta T cell number ( J:143006 )

• the percentage of CD4⁺ T cells in the spleen is reduced to less than a third of that in wild-type

increased CD8-positive, alpha-beta T cell number ( J:143006 )

• there is a 10-fold increase in the percentage of CD8⁺ single positive thymocytes

• the percentage of CD8⁺ T cells among splenocytes is more than double that of controls

absent gamma-delta T cells ( J:143006 )

• gamma-delta T cells are virtually absent in these mice

hematopoietic system

thymus hypoplasia ( J:143006 )

• cellularity of the thymus is reduced by about 4-fold

abnormal T cell differentiation ( J:143006 )

• the pre-arranged TCR genes are expressed abnormally early with intracellular TCR-beta chains detectable in the 75% of the common lymphoid progenitors in the bone marrow

• these TCR-beta chains were also detectable in 35% of multipotent progenitors, 12% of short-term hemopoietic stem cells, and 5% of long-term hemopoietic stem cells of the bone marrow

abnormal double-negative T cell morphology ( J:143006 )

• there is a severe reduction in the percentage of thymocytes at the DN1-3 stages

• however, there is a large population of unusual double-negative thymocytes that are CD3⁺

• this double-negative CD3⁺ population is rarely found in the spleen

decreased double-positive T cell number ( J:143006 )

• there is a greater than 4-fold reduction in the percentage of double-positive thymocytes

• the double-positive thymus makes up 83% of the normal thymus but only 16% in these mutants

decreased CD4-positive, alpha-beta T cell number ( J:143006 )

• the percentage of CD4⁺ T cells in the spleen is reduced to less than a third of that in wild-type

increased CD8-positive, alpha-beta T cell number ( J:143006 )

• there is a 10-fold increase in the percentage of CD8⁺ single positive thymocytes

• the percentage of CD8⁺ T cells among splenocytes is more than double that of controls

absent gamma-delta T cells ( J:143006 )

• gamma-delta T cells are virtually absent in these mice

endocrine/exocrine glands

thymus hypoplasia ( J:143006 )

• cellularity of the thymus is reduced by about 4-fold