Phenotypes Associated with This Genotype

Genotype
MGI:3833957

• Allelic Composition: Eno2^tm1(DTA)Hsak/Eno2⁺; Gng7^{tm2(cre/PGR)Mmsh}/Gng7⁺
• Genetic Background: involves: C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Ablation of medium-spiny projection neurons in the striatum of Gng7^{tm2(cre/PGR)Mmsh}/Gng7⁺ Eno2^tm1(DTA)Hsak/Eno2⁺ mice

nervous system

increased neuron apoptosis ( J:145152 )

• strong TUNEL staining is seen in the caudate putamen, nucleus accumbens and olfactory tubercle 10 days after RU-486 injection

• 13 days after RU-486 injection the density of NeuN positive neurons is dramatically reduced in the caudate putamen and the number of NeuN positive neurons is reduced in the nucleus accumbens core and shell

abnormal medium spiny neuron morphology ( J:145152 )

• lack calbindin positive neurons in the striatum after RU-486 treatment

behavior/neurological

behavior/neurological phenotype ( J:145152 )

N • despite ablation of striatal neurons, no defects in motor coordination are detected

N • all behavioral phenotype data are from mice at 13 to 22 days after RU-486 treatment

abnormal learning/memory/conditioning ( J:145152 )

• treatment with RU-486 after long term memory formation and then testing for memory retention 14 days later shows an impairment in fear memory retention

abnormal cued conditioning behavior ( J:145152 )

• impaired conditioning when using a low intensity unconditioned stimulus but not when using a higher intensity unconditioned stimulus

• impaired development of long term but not of short term fear memory when using a low intensity unconditioned stimulus

impaired cued conditioning behavior ( J:210178 )

• 14 days after RU-486 treatment

• reduced freezing response on 2nd day in conditioning chamber

• unconditioned stimulus is a foot shock

• if RU-486 treatment occurs more than 24 hours after the unconditioned stimulus, fear memory is unaffected

abnormal motor learning ( J:145152 )

• fail to show any improvement in performance over repeated trial on an accelerating rotarod

hyperactivity ( J:145152 )

• increase in activity in an open field assay

cellular

increased neuron apoptosis ( J:145152 )

• strong TUNEL staining is seen in the caudate putamen, nucleus accumbens and olfactory tubercle 10 days after RU-486 injection

• 13 days after RU-486 injection the density of NeuN positive neurons is dramatically reduced in the caudate putamen and the number of NeuN positive neurons is reduced in the nucleus accumbens core and shell