immune system
• mice lack CD8a+ dendritic cell subsets
• bone marrow chimera experiments indicate a lack of CD8a+ DCs is due to a cell-intrinsic effect of the mutant locus
• in vitro bone marrow differentiation cultures fail to generate CD24+ Sirp-alpha- DCs that are similar to CD8a+ DC
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• mice lack splenic CD11chiCD8a+DEC205+ dendritic cells
• mice have a loss of CD11chiCD11bdull DCs and CD11chi CD8a+CD24+ DCs
• mice have normal populations of CD4+ and CD8a-CD4- conventional DC subsets
• lymph nodes and thymi lack CD8a+ DC subsets
• CD103-+DEC205+CD8a-CD11blo/- dermal DC numbers are reduced 5-fold in draining lymph nodes of the skin
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• mice fail to develop West Nile Virus (WNV)-specific memory CD8+ T cells after infection with the virus
• the number of CD8+CD44hiCD62Llowcells memory cells is about half-that of wild-type mice before infection and is five-fold less after infection
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• transplanted syngenic fibrosarcomas grow in size instead of being reapidly rejected as happens in control mice
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• CD8+ T cells from mice infected with West Nile virus (WNV) have defective IFN-gamma secretion when cultured with WNV-peptide
• bone marrow chimera studies demonstrate that defective cytolytic activity is not intrinsic to the CD8+ T cells but rather defective dendritic cell function
• infiltrating CD8+ T cells fail to infiltrate transplanted syngenic tumors
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• CD8+ T cells from mice infected with West Nile virus (WNV) have defective killing of WNV peptide-loaded target cells
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• conventional DCs fail to secrete IL-12 after TL3 stimulation but have normal responses to TLR4 and TLR9 stimulation
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• conventional DCs are unable to cross-present (i.e. take up protein, process into peptide and present) ovalbumin antigen to ova-specific CD8+ T cells
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hematopoietic system
• mice lack CD8a+ dendritic cell subsets
• bone marrow chimera experiments indicate a lack of CD8a+ DCs is due to a cell-intrinsic effect of the mutant locus
• in vitro bone marrow differentiation cultures fail to generate CD24+ Sirp-alpha- DCs that are similar to CD8a+ DC
|
• mice lack splenic CD11chiCD8a+DEC205+ dendritic cells
• mice have a loss of CD11chiCD11bdull DCs and CD11chi CD8a+CD24+ DCs
• mice have normal populations of CD4+ and CD8a-CD4- conventional DC subsets
• lymph nodes and thymi lack CD8a+ DC subsets
• CD103-+DEC205+CD8a-CD11blo/- dermal DC numbers are reduced 5-fold in draining lymph nodes of the skin
|
• mice fail to develop West Nile Virus (WNV)-specific memory CD8+ T cells after infection with the virus
• the number of CD8+CD44hiCD62Llowcells memory cells is about half-that of wild-type mice before infection and is five-fold less after infection
|
• CD8+ T cells from mice infected with West Nile virus (WNV) have defective IFN-gamma secretion when cultured with WNV-peptide
• bone marrow chimera studies demonstrate that defective cytolytic activity is not intrinsic to the CD8+ T cells but rather defective dendritic cell function
• infiltrating CD8+ T cells fail to infiltrate transplanted syngenic tumors
|
• CD8+ T cells from mice infected with West Nile virus (WNV) have defective killing of WNV peptide-loaded target cells
|
cellular
• mice lack CD8a+ dendritic cell subsets
• bone marrow chimera experiments indicate a lack of CD8a+ DCs is due to a cell-intrinsic effect of the mutant locus
• in vitro bone marrow differentiation cultures fail to generate CD24+ Sirp-alpha- DCs that are similar to CD8a+ DC
|