Phenotypes for Zfp568 MGI:3808712 MGI Mouse

abnormal embryo development ( J:168015 )

• the severity of extraembryonic defects correlate to each other but not to embryonic tissue defects

• tetraploid complementation derived embryos display embryonic and extraembryonic tissue defects similar to homozygous mutants

failure of initiation of embryo turning ( J:139139 )

abnormal germ layer development ( J:139139 )

short rostral-caudal axis ( J:139139 )

• a short anterior-posterior axis

embryonic growth arrest ( J:139139 )

• arrest by E9.0

abnormal anterior definitive endoderm morphology ( J:139139 )

• failure of the definitive endoderm to undergo convergent extension during axis elongation at E8.5 to E9.0

• wide definitive endoderm

abnormal lateral plate mesoderm morphology ( J:139139 )

• lateral plate mesoderm is located further away from the midline

• the lateral plate mesoderm is shorter and wider

abnormal paraxial mesoderm morphology ( J:139139 )

• somites are located further away from the midline

abnormal neural tube morphology ( J:139139 )

• headfold failed to fuse to form a neural tube

incomplete rostral neuropore closure ( J:139139 )

• neural tube failed to close normally at some location in 45%

open neural tube ( J:139139 )

• complete failure to close neural tube in 55%

abnormal notochord morphology ( J:139139 )

• notochord was disrupted and was wider in some regions but narrower or absent in other positions

• notochord remained as part of the mesodermal layer at E8.5

abnormal somite development ( J:139139 )

• showed condensed somites that were mediolaterally expanded and narrow in the anterior-posterior axis

• only 15% of homozygous mutants showed normal somites

abnormal extraembryonic tissue morphology ( J:168015 )

abnormal vitelline vasculature morphology ( J:168015 )

• the extraembryonic vascular plexus fails to extend throughout the yolk sac and remains restricted to the blood island region

small allantois ( J:168015 )

• fails to extend at E8.5

abnormal chorion morphology ( J:168015 )

• most have enlarged chorions

• in some the chorion is expanded laterally and contacts the visceral endoderm

• in some cases the chorion forms an expanded ectoplacental cavity

abnormal placenta morphology ( J:168015 )

absent placental labyrinth ( J:168015 )

excessive folding of visceral yolk sac ( J:168015 )

• displays bubble-like protrusions at E7.5 and is noticeably ruffled at E8.5

abnormal extraembryonic tissue physiology ( J:168015 )

• expression analysis indicates defects in signaling between the visceral endoderm and extraembryonic mesoderm

abnormal extraembryonic endoderm formation ( J:168015 )

• in some areas cells are detached and in other areas cells are compressed

abnormal extraembryonic mesoderm development ( J:168015 )

• cells clump at the embryonic-extraembryonic junction in most embryos

failure of chorioallantoic fusion ( J:168015 )

abnormal digestive system development ( J:139139 )

• an open gut tube