Phenotypes Associated with This Genotype

Genotype
MGI:3793301

• Allelic Composition: Tg(IghelMD4)4Ccg/Tg(IghelMD4)4Ccg
• Genetic Background: NOD.B6-Tg(IghelMD4)4Ccg/DvsJ

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal T cell proliferation ( J:80859 )

• T cells fail to proliferate when splenocytes are cultured in vitro with the diabetes autoantigen GAD

decreased CD8-positive, alpha-beta T cell number ( J:80859 )

• the number of CD8 T cells found in the spleen is half that of non-transgenic NOD mice

abnormal mature B cell morphology ( J:80859 , J:89156 )

• the number but not the proportion of B cells found in the spleen is twice that of non-transgenic NOD mice (J:80859)

• 99.3% of these B cells express the transgenic IgM B cell receptor specific for hen egg lysozyme (J:80859)

• 8.4% of the B cells express endogenous IgM indicating there is some escape from allelic exclusion (J:80859)

• 98% of the B cells express the transgenic antibody that is specific for hen egg lysozyme (HEL) (J:89156)

• 94% of these B cells retain the ability to bind HEL protein (J:89156)

increased susceptibility to autoimmune diabetes ( J:80859 )

• 90% of female mice are diabetic by 30 weeks of age which is similar to the incidence rate in non-transgenic NOD mice

• there is a significant delay in time of onset compared to non-transgenic diabetic mice

hematopoietic system

abnormal T cell proliferation ( J:80859 )

• T cells fail to proliferate when splenocytes are cultured in vitro with the diabetes autoantigen GAD

decreased CD8-positive, alpha-beta T cell number ( J:80859 )

• the number of CD8 T cells found in the spleen is half that of non-transgenic NOD mice

abnormal mature B cell morphology ( J:80859 , J:89156 )

• the number but not the proportion of B cells found in the spleen is twice that of non-transgenic NOD mice (J:80859)

• 99.3% of these B cells express the transgenic IgM B cell receptor specific for hen egg lysozyme (J:80859)

• 8.4% of the B cells express endogenous IgM indicating there is some escape from allelic exclusion (J:80859)

• 98% of the B cells express the transgenic antibody that is specific for hen egg lysozyme (HEL) (J:89156)

• 94% of these B cells retain the ability to bind HEL protein (J:89156)

cellular

abnormal T cell proliferation ( J:80859 )

• T cells fail to proliferate when splenocytes are cultured in vitro with the diabetes autoantigen GAD

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
type 1 diabetes mellitus		DOID:9744	OMIM:222100	J:80859