About   Help   FAQ
Phenotypes Associated with This Genotype
Genotype
MGI:3789638
Allelic
Composition
Tg(Cd4-TGFBR2)16Flv/?
Genetic
Background
involves: C3H * C57BL/6 * C57BL/10 * C57BR/cd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Cd4-TGFBR2)16Flv mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• in vitro stimulated CD4- and CD8- T cells are refractory to the inhibitory effects of TGF-beta1 and TGF-beta3 on T cell proliferation
• there is only a slight inhibition of proliferation on activated T cells by TGF-beta2

growth/size/body
• starting about 3 to 4 months of age, mice begin wasting due to an autoimmune disease

immune system
• thymuses of 5 month old mice are completely involuted with only thymic lymph nodes detectable in the area where the thymus in normally present
• there is a decrease in the percentage of B cells found in the spleen though due to the increase in spleen cellularity the total number of B cells is normal
• 90% of the T cells found in the spleen of 5 month old mice are CD62Llow CD44high indicating an activated/memory phenotype
• increases in memory T cell numbers are found as early as 4 weeks of age
• total cell number in the spleen is increased by about 60%
• sera levels of IgA are 16 fold higher in 5-6 month old mice than in age matched wild-type mice
• sera levels of IgG1 are 8 fold higher in 5-6 month old mice than in age matched wild-type mice
• sera levels of IgG2a are 4 fold higher in 5-6 month old mice than in age matched wild-type mice
• all CD8 T cells and most CD4 T cells are in an activated, effector state with CD8 T cells producing IFN-gamma and CD4 T cells producing Th1 and/or Th2 cytokines
• in vitro stimulated CD4- and CD8- T cells are refractory to the inhibitory effects of TGF-beta1 and TGF-beta3 on T cell proliferation
• there is only a slight inhibition of proliferation on activated T cells by TGF-beta2
• there is a 3-fold increase in the number of cells found in the lymph nodes
• auto-antibodies to nuclear proteins are detected in the sera of mice with specificity of these antibodies differing between individual mice
• pronounced inflammation in the gut is evident in 5 month old mice with dense infiltrates of lymphocytes, macrophages and plasma cells occurring in the lamina propia and mucosal epithelia
• the inflammation also causes distortion of the crypt architecture, crypt abscesses and compensatory epithelial hyperplasia
• mild inflammation is also evident in the stomach and duodenum
• mild inflammation is evident in the pancreas of 5 month old mice
• there are clusters of macrophages and lymphocytes located in the parenchyma and adjacent to blood vessels in the liver of 5 month old mice
• IgG deposits are found in the kidney glomeruli of 5 month old mice
• mild infiltration of inflammatory cells is also observed
• lungs of 5 month old mice have focal perivascular and intraalveolar infiltration of lymphocytes and macrophages

digestive/alimentary system
• mice start developing diarrhea at about 3 to 4 months of age
• pronounced inflammation in the gut is evident in 5 month old mice with dense infiltrates of lymphocytes, macrophages and plasma cells occurring in the lamina propia and mucosal epithelia
• the inflammation also causes distortion of the crypt architecture, crypt abscesses and compensatory epithelial hyperplasia
• mild inflammation is also evident in the stomach and duodenum

endocrine/exocrine glands
• thymuses of 5 month old mice are completely involuted with only thymic lymph nodes detectable in the area where the thymus in normally present
• mild inflammation is evident in the pancreas of 5 month old mice

hematopoietic system
• thymuses of 5 month old mice are completely involuted with only thymic lymph nodes detectable in the area where the thymus in normally present
• there is a decrease in the percentage of B cells found in the spleen though due to the increase in spleen cellularity the total number of B cells is normal
• 90% of the T cells found in the spleen of 5 month old mice are CD62Llow CD44high indicating an activated/memory phenotype
• increases in memory T cell numbers are found as early as 4 weeks of age
• total cell number in the spleen is increased by about 60%
• sera levels of IgA are 16 fold higher in 5-6 month old mice than in age matched wild-type mice
• sera levels of IgG1 are 8 fold higher in 5-6 month old mice than in age matched wild-type mice
• sera levels of IgG2a are 4 fold higher in 5-6 month old mice than in age matched wild-type mice
• all CD8 T cells and most CD4 T cells are in an activated, effector state with CD8 T cells producing IFN-gamma and CD4 T cells producing Th1 and/or Th2 cytokines
• in vitro stimulated CD4- and CD8- T cells are refractory to the inhibitory effects of TGF-beta1 and TGF-beta3 on T cell proliferation
• there is only a slight inhibition of proliferation on activated T cells by TGF-beta2

liver/biliary system
• there are clusters of macrophages and lymphocytes located in the parenchyma and adjacent to blood vessels in the liver of 5 month old mice

renal/urinary system
• IgG deposits are found in the kidney glomeruli of 5 month old mice
• mild infiltration of inflammatory cells is also observed

respiratory system
• lungs of 5 month old mice have focal perivascular and intraalveolar infiltration of lymphocytes and macrophages


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer & Copyright Notice
Send questions and comments to User Support.
last database update
10/08/2019
MGI 6.14
The Jackson Laboratory