mortality/aging
• no live embryos are recovered at E9.5 and most have been resorbed at E12.5, with inheritance of the maternally derived mutant allele
• same phenotype is observed when EIIa-cre transgenic males are used
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embryo
• after E7.5, apoptotic nuclei are observed in the trophectoderm
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• at E8.5 and E9.5, embryos appear morphologically disorganized relative to wild-type
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• at E8.5 or E9.5, embryos do not show signs of axial rotation
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• after E7.5, embryos become developmentally growth arrested
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• at E8.5 and E9.5, embryos are visibly smaller than littermates
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• at E8.5 and E9.5, degeneration within the trophectoderm becomes more extensive
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growth/size/body
• at E8.5 and E9.5, embryos are visibly smaller than littermates
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reproductive system
• females with the deletion on the paternally-inherited allele are unable to transmit the deletion to offspring; only homozygous Dkc1+, cre transgene-negative male and female offspring are obtained when females heterozygous for the deleted allele are crossed to wild-type males
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cellular
• after E7.5, apoptotic nuclei are observed in the trophectoderm
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