Phenotypes Associated with This Genotype

Genotype
MGI:3712456

• Allelic Composition: Ncf4^tm1Pth/Ncf4^tm2Pth
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

immune system phenotype ( J:119217 )

N • blood cell counts are normal and bone marrow derived neutrophils (BMNs) exhibit normal differentiated phenotypes with respect to morphology and phagocytosis of bacteria, zymosan and IgG-latex beads

abnormal neutrophil physiology ( J:119217 )

• in response to formyl-methionylleucylphenylalanine (fMLP), total accumuation of reactive oxygen species (ROS) is reduced by ~19% compared to Ncf4^tm1Pth /+ heterozygotes

• intracellular ROS production in response to phagocytosis of IgG-latex beads is reduced by ~42% in primed compared to Ncf4^tm1Pth /+ heterozygotes

• intracellular ROS production in response to S. aureus phagocytosis in primed BMN cells is reduced ~60% compared to Ncf4^tm1Pth /+ heterozygotes

• initial phase of intracellular ROS formation in response to phorbyl myristate acetate (PMA) is reduced by almost half compared to heterozygous mice

increased susceptibility to bacterial infection ( J:119217 )

• mice have significant defect in ability to clear a S. aureus infection in vivo

hematopoietic system

abnormal neutrophil physiology ( J:119217 )

• in response to formyl-methionylleucylphenylalanine (fMLP), total accumuation of reactive oxygen species (ROS) is reduced by ~19% compared to Ncf4^tm1Pth /+ heterozygotes

• intracellular ROS production in response to phagocytosis of IgG-latex beads is reduced by ~42% in primed compared to Ncf4^tm1Pth /+ heterozygotes

• intracellular ROS production in response to S. aureus phagocytosis in primed BMN cells is reduced ~60% compared to Ncf4^tm1Pth /+ heterozygotes

• initial phase of intracellular ROS formation in response to phorbyl myristate acetate (PMA) is reduced by almost half compared to heterozygous mice