Phenotypes Associated with This Genotype

Genotype
MGI:3703880

• Allelic Composition: Smad2^tm1.1Epb/Smad2^tm1.1Epb; Speer6-ps1^{Tg(Alb-cre)21Mgn}/Speer6-ps1⁺
• Genetic Background: involves: 129/Sv * C57BL/6 * DBA * SJL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

liver/biliary system

liver/biliary system phenotype ( J:119642 )

N • mice exhibit normal postnatal liver and body growth and function

abnormal hepatocyte morphology ( J:119642 )

• after attachment to culture dishes, hepatocytes are elongated with process formation and cell separation compared to wild-type cells which have a cobblestone epithelial morphology

abnormal hepatocyte physiology ( J:119642 )

• hepatocytes express much higher levels of vimentin (mesenchymal marker) compared to control or Smad3 mutant cells

increased hepatocyte proliferation ( J:119642 )

• in culture, untreated cells exhibit ~3-fold greater proliferation compared to controls and Smad3 hepatocytes; after TGFbeta treatement, cells show reduced proliferation compared to controls

• in CCL4-injured livers, cell proliferation is higher at earlier time points than in controls or Smad3-deficient livers

cellular

abnormal cell differentiation ( J:119642 )

• hepatocytes dedifferentiate spontaneously to acquire mesenchymal and promigratory features in culture

abnormal cell migration ( J:119642 )

• compared to wild-type cells which cannot migrate to the wound area in a scratch-wound in vitro assay after 24 hours or only partial cover area when treated with TGFbeta, mutant cells cover the entire scratch area in absence of TGFbeta

increased hepatocyte proliferation ( J:119642 )

• in culture, untreated cells exhibit ~3-fold greater proliferation compared to controls and Smad3 hepatocytes; after TGFbeta treatement, cells show reduced proliferation compared to controls

• in CCL4-injured livers, cell proliferation is higher at earlier time points than in controls or Smad3-deficient livers