Phenotypes Associated with This Genotype

Genotype
MGI:3702328

• Allelic Composition: Mesp1/Mesp2^tm1Ysa/Mesp1/Mesp2^tm1Ysa
• Genetic Background: involves: C57BL/6 * CBA * ICR

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:62984 )

• die around E9.5

growth/size/body

decreased embryo size ( J:62984 )

• smaller at E7.5, especially in the anterior region

embryo

abnormal gastrulation ( J:62984 )

• dense cell accumulation in the primitive streak indicates the initiation of gastrulation and the ingression of ectodermal cells into the primitive streak, but the mesodermal cells are unable to depart from the primitive streak

abnormal ectoderm development ( J:62984 )

• embryonic ectoderm expresses only anterior neuronal markers at E9.5

abnormal endoderm development ( J:62984 )

• marker analysis indicates a lack of active migration of the definitive endoderm

abnormal mesoderm development ( J:62984 )

• analysis of molecular markers indicates a lack of the cranio-cardiac and paraxial mesoderm, however the axial mesoderm is initially generated but anterior extension is halted after E8.5

decreased embryo size ( J:62984 )

• smaller at E7.5, especially in the anterior region

absent paraxial mesoderm ( J:62984 )

• lack of paraxial mesoderm

absent mesoderm ( J:62984 )

• lack the mesodermal layer between the endoderm and ectoderm at E7.5

abnormal primitive streak formation ( J:62984 )

• abnormal accumulation of cells in the primitive streak indicates an inability of mesodermal cells to migrate out of the streak

absent somites ( J:62984 )

• do not develop somites

cardiovascular system

abnormal blood vessel morphology ( J:62984 )

• develop endothelial cells indicating that the initiation of vasculogenesis occurs, however no distinct blood vessels are seen

absent heart ( J:62984 )

• do not develop a heart