Phenotypes Associated with This Genotype

Genotype
MGI:3693360

• Allelic Composition: Tg(tetO-BCR/ABL1)27Dgt/0; Tg(MMTVtTA)1Mam/0
• Genetic Background: involves: C57BL/6 * FVB/N * SJL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

premature death ( J:72377 )

• upon withdrawal of tetracycline (TET), expression of BCR/ABL results in development of lethal leukemia; 100% of bitransgenic mice die by ~76 days

hematopoietic system

enlarged spleen ( J:72377 )

• spleen becomes massively enlarged when TET treatment is stopped

anemia ( J:72377 )

• severe anemia develops in peripheral blood without TET treatment

thrombocytopenia ( J:72377 )

• severe thrombocytopenia in peripheral blood develops when TET treatment is stopped

increased leukocyte cell number ( J:72377 )

• increased peripheral blood leukocyte count is observed 10-20 days after TET withdrawal; blood shows presence of cells resembling immature lymphocytes

• when TET is given to mice in advanced stages of disease, WBC counts normalize in 48-72 hours, lymphoblasts are not detected in peripheral blood

immune system

enlarged spleen ( J:72377 )

• spleen becomes massively enlarged when TET treatment is stopped

increased leukocyte cell number ( J:72377 )

• increased peripheral blood leukocyte count is observed 10-20 days after TET withdrawal; blood shows presence of cells resembling immature lymphocytes

• when TET is given to mice in advanced stages of disease, WBC counts normalize in 48-72 hours, lymphoblasts are not detected in peripheral blood

enlarged lymph nodes ( J:72377 )

• nodes become massively enlarged when TET is stopped; when TET is given to mice in advanced stages of disease, complete regression of enlarged lymph nodes occurs within 5 days

cellular

increased apoptosis ( J:72377 )

• resumption of TET treatment results in ~80% of leukemic cells undergoing apoptosis

skeleton

abnormal bone marrow morphology ( J:72377 )

• bone marrow is pale; hematopoietic cells are replaced by lymphoblasts

neoplasm

increased leukemia incidence ( J:72377 )

• 100% of mice develop acute lymphoblastic leukemia (ALL) upon withdrawal of tetracycline administration

• leukocyte counts range from 80000-150000/ul

• leukemic cells infiltrate skin, pleura, and meninges

growth/size/body

enlarged spleen ( J:72377 )

• spleen becomes massively enlarged when TET treatment is stopped