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Phenotypes Associated with This Genotype
Genotype
MGI:3689378
Allelic
Composition
Cftrtm1Kth/Cftrtm1Kth
Genetic
Background
B6.129S7-Cftrtm1Kth
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cftrtm1Kth mutation (1 available); any Cftr mutation (31 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
respiratory system
• increased inflammatory response to chronic Pseudomonas aeruginosa infection, identical to that in Cftrtm1Unc homozygotes
• after treatment with a chloride-depleted solution with amiloride and forskolin, the nasal potential difference (PD) was significantly different between control mice and homozygous mice

mortality/aging
• reduced ability to respond to lung infection elicited with Pseudomonas aeruginosa- laden agarose beads

growth/size/body
• weighed significantly less (P 0.05) than homozygote wild-type controls at 7, 14, and 21 days of age (J:112450)
• at 6-8 and 14-16 weeks of age, total body weight is reduced by only 15% and 12%, respectively, thus not explaining the larger differences noted in average weight of reproductive organs (~50% and 36%, respectively) (J:145380)

digestive/alimentary system
• develop intestinal blockage when fed a normal (solid) diet

immune system
• increased inflammatory response to chronic Pseudomonas aeruginosa infection, identical to that in Cftrtm1Unc homozygotes
• reduced ability to respond to lung infection elicited with Pseudomonas aeruginosa- laden agarose beads

reproductive system
• female homozygotes show an average of 1.5 +/- 2.0 corpora lutea per ovary vs 9.3 +/- 1.4 in wild-type females, even though other follicle stages are present
• at 7 weeks of age, female homozygotes display smaller ovaries than wild-type females
• at 6-8 and 14-16 weeks of age, the average weight of mutant ovaries is reduced by 50% and 36%, respectively, relative to that of wild-type ovaries
• however, mutant ovarian weight is restored to wild-type values after superovulation
• only 1 of 15 female homozygotes showed cervical mucus accumulation with no other physical signs of obstruction in the uterus
• at 7 weeks of age, female homozygotes display smaller uteri than wild-type females
• at 6-8 and 14-16 weeks of age, the average weight of mutant uteri is reduced by 56% and 36%, respectively, relative to that of wild-type uteri
• however, mutant uterus weight is restored to wild-type values after superovulation
• mutant uteri are thinner than wild-type
• female homozygotes display a delayed onset of puberty relative to wild-type controls
• female homozygotes display reduced oocyte ovulation rates relative to wild-type females
• however, normal ovulation rates are observed after induction of superovulation with exogenous hormone (PMSG + hCG) injections
• unlike wild-type females, 22.2% of 14-16-wk-old mutant females never enter estrus but are constantly in diestrus
• at 14-16 weeks of age, female homozygotes that display at least one estrous cycle show half as many cycles as wild-type females, resulting in a 2-fold increase in average cycle length
• female homozygotes exhibit reduced fertility with significantly fewer numbers of litters and smaller litter sizes relative to wild-type females
• 35.7% of female homozygotes are unable to give birth over a 5-month mating period
• following induction of superovulation, only 1 of 10 mutant females that displayed vaginal plugs gave birth, but that female did give birth to 20 pups
• female homozygotes show a significant decrease in average number of pups per litter relative to wild-type females (3.81 +/- 1.43 vs 6.56 +/- 2.36, respectively)
• at 48 hrs after hCG treatment, 98.4% of mutant oocytes remain unfertilized, whereas the majority of embryos from superovulated wild-type females are found at the 2- to 4-cell stages
• however, no significant differences in in vitro fertilization rates are observed, suggesting that decreased in vivo fertilization is more likely due to inadequate fluid control in the reproductive tract, resulting in decreased sperm number in the oviduct
• sperm transport within the mutant female reproductive system is significantly impaired: the average number of sperm found in mutant oviducts is only ~10% that of wild-type
• however, no differences in capacitation of oviductal sperm from mutant and wild-type females are observed

endocrine/exocrine glands
• female homozygotes show an average of 1.5 +/- 2.0 corpora lutea per ovary vs 9.3 +/- 1.4 in wild-type females, even though other follicle stages are present
• at 7 weeks of age, female homozygotes display smaller ovaries than wild-type females
• at 6-8 and 14-16 weeks of age, the average weight of mutant ovaries is reduced by 50% and 36%, respectively, relative to that of wild-type ovaries
• however, mutant ovarian weight is restored to wild-type values after superovulation

homeostasis/metabolism
N
• although mutant FSH levels are slightly increased relative to wild-type levels, circulating levels of both FSH and LH still remain within normal range at proestrus

Mouse Models of Human Disease
OMIM ID Ref(s)
Cystic Fibrosis; CF 219700 J:112450


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
08/23/2016
MGI 6.05
The Jackson Laboratory