Phenotypes Associated with This Genotype

Genotype
MGI:3664097

• Allelic Composition: Ptpn1^tm2Bbk/Ptpn1^tm2Bbk; Tg(Nes-cre)1Kln/0
• Genetic Background: involves: 129S4/SvJae * C57BL/6 * SJL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

growth/size/body

decreased body weight ( J:111969 )

• male neuronal Ptpn1-deficient mice show significantly reduced body weight on a high fat diet compared to control Ptpn1-sufficient (undeleted Ptpn1^tm2Bbk homozygous) animals between 9 and 17 weeks of age; females show reduced body weight but less markedly so than control female mice on a high fat diet

• neuronal Ptpn1-deficient mice also weigh less than controls when fed a regular chow diet (decrease of 4.5% fat by weight)

decreased susceptibility to diet-induced obesity ( J:179621 )

• when fed a high-fat diet

homeostasis/metabolism

abnormal homeostasis ( J:179621 )

• whether fed standard chow or a high-fat diet, leptin-treated mice exhibit enhanced leptin sensitivity compared with wild-type mice

decreased susceptibility to diet-induced obesity ( J:179621 )

• when fed a high-fat diet

decreased circulating glucose level ( J:111969 )

• mutants have lower fed blood glucose on regular chow and HFD vs controls

increased oxygen consumption ( J:111969 )

decreased respiratory quotient ( J:111969 )

• mice display greater oxygen consumption and a decreased respiratory quotient

improved glucose tolerance ( J:111969 )

• male mice at 8-10 weeks of age show enhanced glucose tolerance compared to littermate comtrols

increased insulin sensitivity ( J:111969 , J:179621 )

• male mice at 8-10 weeks of age show improved insulin sensitivity compared to littermate controls (J:111969)

• female mice also show significantly improved insulin sensitivity even though weight at 8 weeks of age is the same as controls; insulin sensitivity and glucose tolerance are similar to whole body deficient Ptpn1^tm1Bbk mice (J:111969)

abnormal hormone level ( J:111969 )

• leptin protein is elevated in white adipose tissue of these mice, suggesting that increased serum leptin levels are due to increased adipocyte leptin production

decreased circulating insulin level ( J:111969 )

• mutants have lower seurm insulin levels on regular chow and HFD vs controls

decreased circulating leptin level ( J:179621 )

increased circulating leptin level ( J:111969 )

• neuronal Ptpn1-deficient mice show increased serum leptin levels (2.3 -fold on a regular chow diet, 4.4-fold higher on a HFD) compared to Ptpn1-undeleted littermates, detected 4 weeks after birth

increased circulating adiponectin level ( J:111969 )

• adiponectin levels are elevated and resistin levels are unexpectedly normal in mutants on regular and HFD diets

decreased triglyceride level ( J:111969 )

• mice show reduced adiposity after high-fat feeding based on lower carcass triglyceride content compared to controls

behavior/neurological

abnormal food intake ( J:111969 )

• intake is slightly less in mutants than controls

• when treated with leptin twice per day over a 60 hour period, male mice show enhanced suppression of food intake vs controls

decreased food intake ( J:111969 )

• male mice injected with leptin show decreased food intake during the period of treatment compared to controls

abnormal motor capabilities/coordination/movement ( J:111969 )

• neuronal Ptpn1-deficient mice display increased ambulatory movement vs control

skeleton

decreased bone mineral density ( J:111969 )

• when treated with leptin twice per day over a 60 hour period, male mice show reduced bone mineral density

adipose tissue

abnormal fat pad morphology ( J:111969 )