Phenotypes Associated with This Genotype

Genotype
MGI:3623130

• Allelic Composition: Calcrl^tm1Kmca/Calcrl^tm1Kmca
• Genetic Background: 129S6/SvEvTac-Calcrl^tm1Kmca

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

Generalized edema in Calcrl^tm1Kmca/Calcrl^tm1Kmca, Adm^tm1Unc/Adm^tm1Unc, and Ramp2^tm1Kmca/Ramp2^tm1Kmca mice

mortality/aging

lethality throughout fetal growth and development, complete penetrance ( J:106927 , J:130930 )

• die between E13.5 and E14.5 (J:106927)

• mice die 1 to 2 days after developing edema (J:130930)

cardiovascular system

abnormal vascular smooth muscle morphology ( J:106927 )

• thin vascular smooth muscle walls are seen at E12.5 and E13.5 but not at E11.5 due to a decrease in vascular smooth muscle cell proliferation

vascular smooth muscle hypoplasia ( J:106927 )

abnormal myocardium layer morphology ( J:106927 )

abnormal myocardium compact layer morphology ( J:106927 )

• at E12.5 and E13.5, the compact zone is discontinuous

thin myocardium compact layer ( J:106927 )

disorganized myocardium ( J:106927 )

• myocardium has a disorganized structure and the chamber appears crowded

small heart ( J:106927 )

• by E13.5, heart is about 2/3 the size of wild-type

increased fetal cardiomyocyte apoptosis ( J:106927 )

• by E13.5, see a 6-fold increase in apoptosis throughout the heart, particularly in the ventricular apex, endocardial cushion, and septum

decreased fetal cardiomyocyte proliferation ( J:106927 )

• by E12.5, see a significant reduction in cell proliferation in the ventricles

homeostasis/metabolism

edema ( J:130930 )

• at E12.5, mice exhibit interstitial edema without hemorrhage

intestinal edema ( J:106927 )

• onset of edema is at E12.5, associated with a distended fluid-filled thoracic cavity

hydrops fetalis ( J:106927 )

• the edema formation rapidly progresses so that by E13.5, all embryos exhibit hydrops fetalis

lymphedema ( J:130930 )

immune system

abnormal lymph organ development ( J:130930 )

• at E13.5, the jugular lymph sac is smaller than in wild-type mice despite lymphedema

• proliferation of lymphatic endothelial cells is decreased compared to in wild-type mice

muscle

abnormal vascular smooth muscle morphology ( J:106927 )

• thin vascular smooth muscle walls are seen at E12.5 and E13.5 but not at E11.5 due to a decrease in vascular smooth muscle cell proliferation

vascular smooth muscle hypoplasia ( J:106927 )

abnormal myocardium layer morphology ( J:106927 )

abnormal myocardium compact layer morphology ( J:106927 )

• at E12.5 and E13.5, the compact zone is discontinuous

thin myocardium compact layer ( J:106927 )

disorganized myocardium ( J:106927 )

• myocardium has a disorganized structure and the chamber appears crowded

increased fetal cardiomyocyte apoptosis ( J:106927 )

• by E13.5, see a 6-fold increase in apoptosis throughout the heart, particularly in the ventricular apex, endocardial cushion, and septum

decreased fetal cardiomyocyte proliferation ( J:106927 )

• by E12.5, see a significant reduction in cell proliferation in the ventricles

digestive/alimentary system

intestinal edema ( J:106927 )

• onset of edema is at E12.5, associated with a distended fluid-filled thoracic cavity

integument

abnormal skin condition ( J:106927 )

• distended skin is seen at E13.5, due to fluid accumulation in the interstitial space

cellular

increased fetal cardiomyocyte apoptosis ( J:106927 )

• by E13.5, see a 6-fold increase in apoptosis throughout the heart, particularly in the ventricular apex, endocardial cushion, and septum

decreased fetal cardiomyocyte proliferation ( J:106927 )

• by E12.5, see a significant reduction in cell proliferation in the ventricles